From: Nucleic acid detection in the diagnosis and prevention of schistosomiasis
Biomarkers | Method | Sample type | Sensitivity % | Specificity % | References |
---|---|---|---|---|---|
S. japonicum | |||||
SjR2 (AF412221) | LAMP | human and rabbit serum | 96.7 | 100 | [7] |
SjR2 (AF412221) | PCR | rabbit serum | 60 | 100 | [24] |
SjR2 (AF412221) | Real-time PCR | rabbit serum | — | — | [38] |
SjR2 (AF412221) | Nested PCR | rabbit serum | — | — | [40] |
SjCHGCS19 | Nested PCR | human and rabbit serum | 97.67 | 96.07 | [41] |
28 s rDNA(Z46504) | LAMP | snail tissue | — | — | [14] |
SjCHGC08270 | Real-time PCR | Water | 93 | — | [16] |
5D gene | Nested PCR | adult worm and egg | — | — | [58] |
NADH I | Real-time PCR | human, pig, buffalo feces | — | — | |
Mitochondria gene | PCR | adult worm | — | — | [60] |
ITS2 | Real-time PCR | mouse feces, snail tissue | — | — | [61] |
S. mansoni | |||||
SM1-7 (M61098) | PCR | human feces, urine, serum and mice serum | 96–100 | 88–91.2 | |
SM1-7 (M61098) | PCR | water and plankton | — | — | |
SM1-7 (M61098) | LAMP | snail tissue | — | — | [10] |
SM1-7 (M61098) | PCR-ELISA | human feces | 97.4 | 85.1 | |
SM1-7 (M61098) | Real-time PCR | human feces and serum | 80 | 92.4 | [8] |
Genomic DNA | Hybridization | snail tissue | — | — | [63] |
18 s rDNA | Nested PCR | snail tissue | — | — | [46] |
mitochondria gene | Multiplex PCR | snail tissue | — | — | |
Satellite DNA | LAMP | mice feces and serum | — | — | [72] |
S. haematobium | |||||
Dra I | PCR | adult worm, snail tissue, human brain and urine | 98 | 82 | |
Dra I | Real-time PCR | human serum, urine, feces | 96.6–100 | 93.6–94.4 | [23] |
Dra I | LAMP | snail tissue | — | — | [10] |
Sh110 | PCR | adult worm, snail tissue | — | — | [74] |
Sh73/Dra I | Multiplex PCR | adult worm, snail tissue | — | — | [45] |
NADH3 | PCR | snail tissue | — | — | [55] |
ITS2 (DQ677661) | PCR | human urine | — | — | |
Differential detection methods | |||||
ITS2 (AF146037/8) | PCR-RFLP | adult worm and cercaria | — | — | [75] |
COX I | Multiplex PCR | adult worm | — | — | [76] |
005AAT/Dra I | PCR | adult worm | — | — | [77] |
Dra I/SM1-7 | PCR | human urine, feces | 99–100 | 71–100 | [17] |
COX I | PCR | adult worm, human blood | — | — | |
Mitochondria gene | Copro-PCR | human feces | 87.7 | 100 | [79] |