Open Access

Parasitic contamination of raw vegetables and fruits collected from selected local markets in Arba Minch town, Southern Ethiopia

  • Fitsum Bekele1Email author,
  • Tamirat Tefera2,
  • Gelila Biresaw3 and
  • Tsegaye Yohannes3
Infectious Diseases of Poverty20176:19

DOI: 10.1186/s40249-016-0226-6

Received: 14 July 2016

Accepted: 20 December 2016

Published: 7 March 2017

Abstract

Background

One way that people get infected with intestinal parasites is through the consumption of contaminated vegetables and fruits. This study aimed at determining the prevalence and predictors of parasitic contamination of fruits and vegetables collected from four local markets in Arba Minch town, Southern Ethiopia.

Methods

A cross-sectional study was conducted from 1 to 21 September 2014 to determine the level of parasitic contamination of fruits and vegetables sold in Arba Minch town. A total of 360 samples of different types of fruits and vegetables were soaked in physiological saline, followed by vigorous shaking with the aid of a mechanical shaker for 15 minutes and then examined using the sedimentation concentration technique.

Results

Out of the 360 samples examined, 196 (54.4%) were contaminated with at least one type of parasite. Ascaris lumbricoides (20.83%) was the most frequently detected parasite and Isospora belli (3.06%) was the least frequently detected one. It was also observed that decreased parasitic contamination was significantly associated with washing the products before displaying it for selling (P < 0.001).

Conclusions

The findings of this study provide evidence that there is a potentially high risk of acquiring parasitic infections from the consumption of raw vegetables and fruits in Arba Minch, Ethiopia. The authors believe that an effort should be made by the relevant bodies to reduce the rate of contamination of products with medically important parasites by educating the vendors and the community.

Keywords

Vegetable and fruits contamination Intestinal parasite Health education Arba Minch Ethiopia

Multilingual abstracts

Please see Additional file 1 for translations of the abstract into six official working languages of the United Nations.

Background

Intestinal parasitic infections are widely distributed throughout the world, endangering public health. Infections with medically important parasites (intestinal helminths and protozoa) are closely linked with conditions of poverty, unsafe water, crowded living conditions, lack of sanitation and hygiene [1, 2].

Food borne diseases continue to be a common and serious threat to public health all over the world and these diseases are a major cause of morbidity [3]. Outbreaks of human infections due to the consumption of raw fruits and vegetables have occurred with increased frequency during the past decade [3]. Studies have shown that Ascaris lumbricoides, Cryptosporidium spp., Entamoeba histolytica, Enterobius vermicularis, Fasciola spp., Giardia intestinalis, hookworm, Hymenolepis spp., Taenia spp., Trichuris trichiura, and Toxocara spp., can infect humans who consume contaminated, uncooked, or improperly washed vegetables and fruits [4].

Many studies had been conducted to evaluate the role of raw vegetables in the transmission of intestinal parasites, for example, in Alexandria, Egypt; Tripoli, Libya; Riyadh, Saudi Arabia; Iraq; Tehran and Qazvin Province, Iran; and the Philippines [513]. All have stressed the importance of fruits and vegetables, particularly which are consumed raw and unwashed, in the transmission of medically important parasites.

Fruits and vegetables—by being a source of essential nutrients, vitamins, minerals, proteins, and fibers—play a major role in protecting the human body from a number of diseases. Consuming raw and improperly washed vegetables is a major way in which human pathogens are transmitted [10, 11]. Because of poor hygienic practices related to planting, harvesting, packing, transportation, and storage, fruits and vegetables can become easily contaminated with parasites [11].

In developing countries such as Ethiopia, poor sanitation, and substandard and crowded living conditions lead to an increased risk of acquiring parasitic infections. To the best of our knowledge, there is no published document about the level of parasitological contamination of fruits and vegetables in Arba Minch town, Southern Ethiopia. Therefore, this study was designed to determine the level of parasitic contamination of selected fruits and vegetables and associated factors in this location.

Methods

Study area

A cross-sectional study was conducted between 1 and 20 September, 2014 to determine the level of parasitic contamination of fruits and vegetables sold in selected local markets in Arba Minch town, Southern Ethiopia. Arba Minch is located 505 km south of the capital Addis Ababa at an altitude of 1 200–1 300 meters above sea level with an average annual temperature of 29.7 °C. The town experience two distinct wet seasons which occur from March to May followed by a lesser rainfall season in October to December. The average annual rainfall is 700 mm.

Data collection

A pre-tested semi-structured questionnaire was used for collecting data on factors associated with parasitic contamination of fruits and vegetables such as: status of the produces [washed before display or not, freshly collected or stayed more than one day, source of water used for washing, educational status of the vendors]. Data on means of display and type of the market were recorded by simple observation. In each market, samples were collected under normal purchase conditions from randomly selected sellers. A total of 40 vendors participated in this study.

Sample collection and analysis

Eight types of fruits and vegetables (Persea americana (Avocado), Lactuca serriola (Lettuce), Brassica oleracea (Cabbage), Daucus carota (Carrot), Lycopersicon esculentum (Tomato), Capsicum annuum(Green pepper), Musa paradisiaca (Banana), and Mangifera indica (Mango)) were purchased from four conveniently located local markets, namely, Sikela (1 235 meters above sea level), Secha (1 300 meters above sea level), Yetnebersh (12 90 meters above sea level), and Konso-Sefer (1 275 meters above sea level) in Arba Minch town. An equal number of samples (45 each, total 360 samples) were collected from the markets.

Each sample was placed in a separate plastic bag and labeled with a unique number and its date of collection, and brought to the Arba Minch Hospital Leishmaniasis Research Centre Laboratory for parasitological analysis. Approximately 200 grams of each vegetable or fruit was soaked (for 15 minutes) in one liter of physiological saline, followed by vigorous shaking with the aid of a mechanical shaker [Vortex Genie 2] for 15 minutes. After overnight sedimentation in the washing solution, 15 milliliters of the sediment was transferred to a centrifuge tube using a sieve to remove undesirable matter. For concentrating the parasitic stages (ova, larvae, cysts, and oocysts), the tube was centrifuged at 3000 revolutions per minute for five minutes [13]. After centrifugation, the supernatant was decanted carefully without shaking. Then, the sediment was agitated gently by hand to redistribute the parasitic stages. Finally, the sediment was examined under a light microscope [Olympus CHT] using × 10 and × 40 objectives. Modified Ziehl-Neelsen stained smears were prepared to detect coccidian protozoan oocysts including Cryptosporidium spp., Isospora belli,and Cyclospora cayetanensis [1416].

Data analysis

Statistical analysis was performed with SPSS software version 16 (IBM, Chicago, IL, USA). Probability values were considered to be statistically significant when the calculated P-value was equal to or less than 0.05. The difference in parasitic contamination among the different categories was compared using the Pearson’s chi-square test (χ2) and Fisher’s exact test, where appropriate. Univariate and multivariate logistic regression analyses were performed to identify factors associated with parasitic contamination of the fruits and vegetables.

Results

In this study, 196 samples were identified as being contaminated with at least one type of parasite; the overall contamination rate was 54.4%. Table 1 summarizes what percentage of each sample group was infected: 48.9% of the Capsicum annuum was infected, 66.7% of Brassica oleracea, 37.8% of Persea americana, 60.0% of Lactuca serriola, 62.2% of Daucus carota, 71.1% of Lycopersicon esculentum, 48.9% of Musa paradisiacal, and 40.0% of Mangifera indica.
Table 1

Frequency distribution of parasitological contamination of fruits and vegetables inselected markets inArba Minch town 1 to 22 September 2014

Type of produce

Number examined

Number positive [%]

Number of parasite species detected

One [%]

Two [%]

Three [%]

Four [%]

Capsicum annuum

45

22 [48.9]

16 [35.6]

4 [8.9]

2 [4.4]

0

Mangiferaindica

45

18 [40.0]

14 [31.1]

3 [6.7]

1 [2.2]

0

Perseaamericana

45

17 [37.8]

14 [31.1]

1 [2.2]

1 [2.2]

1 [2.2]

Lycopersiconesculentum

45

32 [71.1]

27 [60.0]

3 [6.7]

1 [2.2]

1 [2.2]

Daucuscarota

45

28 [62.2]

23 [51.1]

3 [6.7]

2 [4.4]

0

Lactucaserriola

45

27 [60.0]

25 [55.6]

2 [4.4]

0

0

Brassica oleracea

45

30 [66.7]

26 [57.8]

3 [6.7]

1 [2.2]

0

Musa paradisiaca

45

22 [48.9]

19 [42.2]

2 [4.4]

1 [2.2]

0

Total

360

196 [54.4]

164 [45.6]

21 [46.7]

9 [20.0]

2 [4.4]

The parasites detected included ova of A. lumbricoides, Toxocara spp., Hymenolepis nana, and H. diminuta; oocysts of Cyclospora, I. belli, and Cryptosporidium spp.; and cysts of G. intestinalis and E. histolytica/dispar. Table 2 shows that A. lumbricoides (20.83%) was the most frequently detected parasite, followed by Toxocara (15.83%), Hymenolepis nana (15.56%), E. histolytica/dispar (14.44%), G. intestinalis (10.0%), H. diminuta (7.78%), Cyclospora (6.94%), Cryptosporidium (4.72%), and I. belli (3.06%).
Table 2

Prevalence of intestinal parasites in fruits and vegetables sold at four local markets in Arba Minch town from 1 to 22 September 2014

Detected parasite

Frequency

Prevalence

Ascaris lumbricoides

75

20.83%

Toxocara spp.

57

15.83%

Hymenolepis nana

56

15.56%

Entamoeba histolytica/dispar

52

14.44%

Giardia intestinalis

36

10.00%

H. diminuta

28

7.78%

Cyclospora spp.

25

6.94%

Cryptosporidium

17

4.72%

Isospora belli

11

3.06%

Contamination with more than one parasite species was observed in the fruit and vegetable samples examined in this study. Almost half (46.7%) of the total samples were contaminated with two species of parasites, while 20% of the samples were contaminated with three species of parasites and quadruple parasitic contamination was observed in 4.4% of the samples. The parasitic contamination rate of the different fruits and vegetables was significantly different (P = 0.03) (see Table 3).
Table 3

Factors associated with parasitic contamination of fruits and vegetables sold in selected markets of Arba Minch town from 1 to 22 September 2014 determined using the chi-square test

Variable

Result of parasitological analysis

Positive [%]

Total

χ2 value

P-value

Educational status of vendors

    

 No formal education

115 [63.9]

180

  

 Primary education

70 [46.7]

150

14.07

0.001

 Secondary education

11 [36.7]

30

  

Total

196 [54.4]

360

  

Market

    

 Secha

50 [55.6]

90

  

 Sikela

48 [53.3]

90

  

 Konso-sefer

60 [66.7]

90

4.98

0.064

 Yetnebersh

38 [42.2]

90

  

Total

196 [54.4]

360

  

Type of produce

    

Lactucaserriola

27 [60]

45

  

Mangiferaindica

18 [40]

45

  

Perseaamericana

17 [37.8]

45

  

Lycopersiconesculentum

32 [71.2]

45

8.79

0.03

Daucuscarota

28 [62.3]

45

  

Capsicum annuum

22 [48.9]

45

  

Musa paradisiaca

22 [48.9]

45

  

Brassica oleracea

30 [66.7]

45

  

Total

196 [54.4]

360

  

Means of display

    

 On the floor

111 [55.5]

200

  

 On tops of tables

29 [39.7]

73

5.66

0.722

 On wheelbarrow

36 [41.4]

87

  

Total

196 [54.4]

360

  

Washed before display

    

 Yes

40 [38.5]

104

  

 No

156 [60.9]

256

6.78

0.001

Total

196 [54.4]

360

  

Source of water for washing

    

 Pipe

21 [35.0]

60

  

 River

14 [48.3]

29

1.771

0.441

 Well

2 [28.6]

7

  

Total

37 [38.5]

96

  

Market type

    

 Grocery

36 [60]

60

  

 Open market

160 [53.3]

300

0.39

0.88

Total

196 [54.4]

360

  
Further analysis with binary logistic regression revealed that, compared to Persea americana, Lycopersicon esculentum was significantly contaminated (adjusted odds radio, AOR = 3.4, 95% confidence interval, CI [1.6, 9.9]) (see Table 4).
Table 4

Binary logistic regression of factors associated with parasitic contamination of fruits and vegetables sold in selected markets of Arba Minch town from 1 to 22 September 2014

Variables

Pos. (%)

Laboratory result for parasitic contamination

COR (95% CI) AOR (95% CI)

Sample type

   

Mangiferaindica

18 [40]

1.0 (0.3, 2.4)

1.0 (0.2, 2.6)

Brassica oleracea

30 [66.7]

2.4 (1.2, 5.8)*

2.2 (1.0, 5.6)

Daucuscarota

28 [62.3]

1.7 (0.7, 4.4)

1.7 (0.7, 4.6)

Capsicum annuum

22 [48.9]

1.3 (0.6, 2.6)

1.3 (0.5, 2.8)

Lactucaserriola

27 [60]

1.8 (0.8, 4.3)

1.8 (0.7, 4.4)

Lycopersiconesculentum

32 [71.2]

2.5 (1.1, 5.9)*

3.4 (1.6, 9.9)

Musa paradisiaca

22 [48.9]

1.2 (0.5, 2.7)

1.2 (0.4, 2.9)

Perseaamericana**

17 [37.8]

  

Washed before display

   

 No

156 [38.5]

3.1 (1.8, 4.4)*

3.6 (1.9, 4.6)

 Yes**

40 [60.9]

  

Market

   

 Secha

50 [55.6]

0.5 (0.3, 1.0)

0.5 (0.3, 0.9)

 Sikela

48 [53.3]

0.6 (0.4, 1.0)

0.5 (0.3, 1.1)

 Konso-Sefer

60 [66.7]

1.1 (0.6, 2.0)

1.1 (0.5, 2.1)

 Yetnebersh**

38 [42.2]

  

Means of display

   

 On the floor

111 [55.5]

1.2 (0.5, 5.1)

1.7 (0.4, 5.1)

 On wheelbarrow

36 [41.4]

1.5 (0.3, 4.9)

1.6(0.4, 5.5)

 On table**

29 [39.7]

  

Market type

   

 Grocery

36 [60]

1.2 (0.6, 1.9)

1.2 (0.6, 2.1)

 Open market

160 [53.3]**

  

Vendor’s educational status

   

 No formal education

115 [63.9]

1.2 (0.6, 2.6)

1.3 (0.6, 2.8)

 Primary education

70 [46.7]

0.9 (0.4, 1.8)

0.9 (0.6, 1.9)

 Secondary education**

11 [36.7]

  

*Significant at P-value of 0.05; **reference category

The results also showed that samples collected from Konso-Sefer (66.7%) had the highest contamination rate, followed by samples collected from Secha (55.6%), Sikela (53.3%), and Yetnebersh (42.2%). However, the contamination rate of samples collected from the different markets was statistically insignificant (P = 0.064) (see Table 3). Samples were collected from both open markets and groceries. Samples from groceries comprised 18.4% of the positive samples, while 81.6% of the contaminated products were from open markets; the difference was not statistically significant (P = 0.88) (see Table 3).

This study also assessed which factors were associated with contamination of fruits and vegetables by conducting interviews with the vendors in the markets. Vendors were asked about their educational status and it was revealed that the majority (50%) of the vendors had no formal education, while 42% had primary education and only 8% had secondary education. There was significant association between the education level of vendors and the parasitic contamination rate of the produce they were selling (P = 0.001) (see Table 3).

Among the factors associated with parasitic contamination of fruits and vegetables is the act of washing products before displaying it for sale. This study showed that the majority (79.6%) of the products were not washed before display, with only 20.4% being washed. The cross tabulation of washing the products before display for sale and the results of the parasitological analysis showed a significant difference in the contamination rate between washed and unwashed products (P = 0.001) (see Table 3). Compared to washed products, the odds of unwashed products becoming contaminated with at least one parasite was 3.6 times higher (AOR = 3.6, 95% CI [1.9, 4.6]) (see Table 4).

The means of display was also assessed for association with parasitic contamination of fruits and vegetables; a statistically insignificant association was observed (P = 0.072).

The sources of water used for washing products were also examined: pipe water (62.5%), well water (30.2%), and river water (7.3%). The analysis revealed that 35%, 48.3%, and 28.6% of the produce washed by pipe water, well water, and river water was contaminated with at least one parasite species, respectively. There was no significant difference observed in the contamination rate between the products washed by water from different sources (P = 0.441) (see Table 3).

Discussion

Like many tropical countries, intestinal parasites are widely distributed in Ethiopia because of the favorable climate and unsanitary conditions that facilitate fecal pollution of water, foodstuffs, and soil [2, 11]. The market chain lends itself to fruits and vegetables passing through several hands and as a result they might become contaminated with enteric bacteria, viruses, and parasitic pathogens [16].

The present study attempted to assess the level of contamination and prevalence of different intestinal parasites in various fruits and vegetables sold in local markets of Arba Minch, Southern Ethiopia. The overall parasitic contamination rate was found to be 54.4%, which is in agreement with other findings from southwest Ethiopia and elsewhere [1115]. It is also, however, higher than that reported in similar studies from, Egypt, Ethiopia and Gaza governorates [5, 1720]. The incongruity between this study and others might be attributed to the variations in geographical locations, climatic and environmental conditions, differences in the sample size, the techniques used, poor post-harvest handling method and socioeconomic status.

Lycopersicon esculentum (71.2%) was found to be the most frequently contaminated product, followed by Brassica oleracea (66.7%), Daucus carota (62.3%), Lactuca serriola (60.0%), Capsicum annuum and Musa paradisiaca (48.9% each).Mangifera indica (40.0%) and Persea americana (37.8%) were found to be the least contaminated. The variation in contamination between the products might be explained by the fact that vegetables such as cabbage, carrot, and lettuce have larger and uneven surfaces, which make the parasites attach more easily to the surface. The smooth surface of Capsicum annuum, Persea americana, and Mangifera indica might reduce the rate of parasitic attachment hence explaining the lower contamination rate observed in this study [5, 21].

In this study, A. lumbricoides was the most frequently detected parasite with a prevalence of 20.83%. The predominance of A. lumbricoides agrees with studies conducted in Philippines and Kenya [11, 21]. This dominance might be associated with this parasite’s ubiquitous distribution, the high number of eggs produced by the fecund female parasite which contributes to the parasite ubiquitous distribution, and the strong and resistant nature of the eggs that enables them to survive unfavorable conditions. The eggs can survive in the absence of oxygen, live for two years at 5–10 °C, and be unaffected by desiccation for two to three weeks [22].

The second most prevalent contaminant was Toxocara species with the prevalence of 15.83%. This dominance might be attributed to the fertility of Toxocara female adults producing up to 10 000 eggs daily and the nature of the eggs, which may survive for up to ten years resisting harsh environmental conditions [23, 24].

H. nana was the third most frequently detected parasite in this study, with a prevalence of 15.56%. This finding is higher than the finding of 8.3% reported in Jimma, Ethiopia and also a study done in Banha, Egypt, which reported a prevalence of 2.8%. The difference observed might be due to difference in climatic conditions and geographical location [15, 25].

In this study, no ova of hookworm are detected. This is in agreement with other studies conducted in Jimma, Ethiopia and Banha, Egypt [15, 25]. It is known that hookworm has a very short life span in the soil and this characteristic might have contributed to its absence in the present study [26, 27]. On the contrary, a study done in Jos, Nigeria has reported the contamination of vegetables with hook worm species [28]. The differences might be attributed to differences in geographical locations, climatic conditions, and types of soil [28, 29].

Various studies support the findings of this study in regards to contamination of fruits and vegetables with G. intestinalis. These include a study done in Tripoli, Libya, and Ardabil, Iran, reporting a prevalence of 10% and 7%, respectively [7, 30]. Our findings on G. intestinalis are also in agreement with a recent study done in Ahar, Iran, which reported a prevalence of 10% [31].

In general, comparing with other similar studies done in the region, a higher rate of parasitic contamination of fruits and vegetables was observed in this study and this could be attributed to many factors such as geographical location, type and number of samples examined, methods used for detection of the intestinal parasites, type of water used for irrigation, and post-harvesting handling methods, which are different from one country to another. In addition to the above factors, individual hygienic habits, sanitary facilitations, and climatic conditions all play a significant role [3].

Contamination with multiple species was observed in all kinds of fruits and vegetables in this study. This might indicate the possibility of a high-level contamination of fruits and vegetables, which perhaps results in multiple parasitic infections in humans. It might also indicate the persistence of intestinal parasitic infections in the area [13].

Even though it was statistically insignificant, the contamination rate was different for the samples collected from different markets. Samples collected from Konso-Sefer showed a higher rate of contamination. This might be associated with the way the products are displayed and the act of washing produce before display. The majority (55.5%) of the samples was displayed on the floor, which exposes them to flies, and 60.9% of the samples were not washed before display. It has been reported that flies can act as a vector for parasites such as Cryptosporidium parvum [32].

The odds of unwashed produce before display becoming contaminated with at least one parasite was 3.6 times higher (AOR = 3.6, 95% CI[1.9, 4.6]) when compared with those washed before display. This might be due to the risk of contamination of the produce during transportation and other post-harvest related activities [15, 32].

Conclusion

The high prevalence of intestinal parasites in the fruits and vegetables consumed in Arba Minch indicates that produce is one of the sources leading to parasitic infections among the public. The authors believe that prevention of contamination remains the most effective way of reducing fruit- and vegetable-borne parasitic infections, and this can be achieved by proper washing of vegetables, improved hygienic practices of vegetable handlers, and improvements in sanitation standards. Comprehensive health education should also be given to vendors and farmers. Vendors should ensure that produce does not make contact with the soil when displaying for selling. In addition, other research must be done to evaluate the level of parasitic contamination of farm products, water, and soil in which fruits and vegetables are cultivated.

Abbreviations

AOR: 

Adjusted odds ratio

CI: 

Confidence interval

COR: 

Crude odds ratio

Declarations

Acknowledgments

We would like to thank Arba Minch Hospital Leishmaniasis Research Laboratory for its contribution. We would also like to extend our gratitude to the vendors for their willingness and cooperation during the study.

Funding

The study was supported by the Arba Minch Hospital Leishmaniasis Research and Treatment Centre. The funders played no role in the study design, data collection, and data analysis or interpretation.

Availability of data and materials

The datasets analyzed during the current study are available from the corresponding author on reasonable request.

Authors’ contributions

Conceived the study: FB. Designed the study: FB, TT, TY. Involved in data collection: FB, TT, GB, TY. Participated in data analysis and interpretation: FB, TT, GB. Drafted the paper: FB. Critically reviewed the paper: TT, GB. All authors read and approved the final paper.

Authors’ information

FB is a lecturer in Medical Parasitology at WolaitaSodo University, Department of Public Health. TT is a lecturer in Medical Parasitology at Jimma University. GB is an assistant lecturer in Medical Laboratory Technology at Arba Minch University. TY is a lecturer in Medical Parasitology at Arba Minch University.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Not applicable.

Ethics approval and consent to participate

The study protocol was reviewed and approved by the Jimma University Ethical Review Board [Rpgc/512/2014]. Data were collected using questionnaires after the purpose of the study was explained to the respondents (vendors of fruits and vegetables) and verbal consent was obtained.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Department of Public Health, College of Health Sciences and Medicine, Wolaita Sodo University
(2)
Department of Medical Laboratory Sciences and Pathology, College of Public Health and Medical Sciences, Jimma University
(3)
Department of Medical Laboratory Science, College of Health Sciences and Medicine Arba Minch University

References

  1. Okyay P, Ertug S, Gultekin B, Onen O, Beser E. Intestinal parasites prevalence and related factors in school children, a western city sample-Turkey. BMC Public Health. 2004;4:64.View ArticlePubMedPubMed CentralGoogle Scholar
  2. Wegayehu T, Tsalla T, Seifu B, Teklu T. Prevalence of intestinal parasitic infections among highland and lowland dwellers in Gamo area, South Ethiopia. BMC Public Health. 2013;13:151.View ArticlePubMedPubMed CentralGoogle Scholar
  3. de W Blackburn C, McClure PJ. Foodborne pathogens: hazards, risk analysis and control. Washington DC: CRC Press; 2002. p. 18–9.View ArticleGoogle Scholar
  4. Kozan E, Gonenc B, Sarimehmetoglu O, Aycicek H. Prevalence of helminth eggs on raw vegetables used for salads. Food Control. 2005;16:239–42.View ArticleGoogle Scholar
  5. Said DE. Detection of parasites in commonly consumed raw vegetables. Alexandria J Med. 2012;48:345–52.View ArticleGoogle Scholar
  6. Hassan A, Farouk H, Abdul-Ghani R. Parasitological contamination of freshly eaten vegetables collected from local markets in Alexandria, Egypt: A preliminary study: a preliminary study. Food Control. 2012;26(2):500–3.View ArticleGoogle Scholar
  7. AbougrainAK NMH, Madi NS, Saied MM, Ghenghesh KS. Parasitological contamination in salad vegetables in Tripoli-Libya. Food Control. 2010;21(5):760–2. doi:10.1016/j.foodcont.2009.11.005.View ArticleGoogle Scholar
  8. Al-Megrin WAI. Prevalence of intestinal parasites in leafy vegetables in Riyadh, Saudi Arabia. Int J Zool Res. 2010;5(2):20–3. doi:10.3923/ijtmed.2010.20.23.Google Scholar
  9. Hadi AM. Isolation and identification of intestinal parasites from vegetables from different markets of Iraq. Bull Iraq Natural History Museum. 2011;11(4):17–25.Google Scholar
  10. Gharavi MJ, Jahani MR, Rokni MB. Parasitic contamination of vegetables from farms and markets in Tehran. Iran J Public Health. 2002;31(4):83–6.Google Scholar
  11. Sia Su GL, Mariano CMR, Matti NSA, Ramos GB. Assessing parasitic infestation of vegetables in selected markets in Metro Manila, Philippines. Asian Pacific J Trop Dis. 2012;2(1):51–4.View ArticleGoogle Scholar
  12. Shahnazi M, Jafari-Sabet M. Prevalence of parasitic contamination of raw vegetables in villages of Qazvin Province, Iran. Foodborne Pathog Dis. 2010;7(9):1025–30. doi:10.1089/fpd.2009.0477.View ArticlePubMedGoogle Scholar
  13. Omowaye OS, Audu PA. Parasites contamination and distribution on fruits and vegetables in Kogi, Nigeria. CIBTech J Bio-Protocols. 2012;1(1):44–7. Available at http://www.cibtech.org/cjbp. Accessed on June 2015.Google Scholar
  14. Idahosa OT. Parasitic Contamination of Fresh Vegetables Sold in Jos Markets. Global J Med Res. 2011;11(1):20–5.Google Scholar
  15. Tefera T, Biruksew A, Mekonnen Z, Eshetu T. Parasitic contamination of fruits and vegetables collected from selected local markets of Jimma town, southwest Ethiopi. Int Sch Res Notices. 2014;2014:382715. doi:10.1155/2014/382715.PubMedPubMed CentralGoogle Scholar
  16. Cheesbrough M. District Laboratory Practice in Tropical Countries, part 1. NY, USA: Cambridge University Press; 2009. p. 206–7.Google Scholar
  17. TomassZ KD. Parasitological contamination of wastewater irrigated and raw manure fertilized vegetables in Mekelle city and its suburb, Tigray, Ethiopia. Momona Ethiopian J Sci. 2012;4(1):77–89.Google Scholar
  18. Al-Shawa RM, Mwafy SN. The enteroparasitic contamination of commercial vegetables in Gaza Governorates. J Infect Dev Countries. 2007;1(1):62–6.Google Scholar
  19. Ishaku A, Ishakeku D, Agwale S. Prevalence of parasitic contamination of some edible vegetables sold at alhamis market in lafiametropolist. Scholar J Biotechnol. 2013;2(2):26–9. Available online at http://www.scholarly-journals.com/SJBISSN 2315-6171 Accessed on Sep 2014.Google Scholar
  20. Ogbolu DO, Alli OA, Ogunleye VF, Olusoga OF, Olaosun I. The presence of intestinal parasites in selected vegetables from open markets in south western Nigeria. Afr J Med Med Sci. 2009;38(4):319–24.PubMedGoogle Scholar
  21. Nyarango RM, Aloo PA, Kabiru EW, Nyanchongi BO. The risk of pathogenic intestinal parasite infections in Kisii Municipality, Kenya. BMC Public Health. 2008;8:237.View ArticlePubMedPubMed CentralGoogle Scholar
  22. Roberts AD. Ascariasis: Introduction and Epidemiology and Transmission. In: Satoskar AR, Simon GL, Hotez PJ, Tsuji M, editors. Medical Parasitology. Austin (Texas): Landes Bioscience; 2009. p. 38–42.Google Scholar
  23. Sunil B, Thomas DR, Latha C, Shameem H. Assessment of parasitic contamination of raw vegetables in Mannuthy, Kerala state, India. Vet World. 2014;7(4):253–6. doi:10.14202/vetworld.2014.253-256.View ArticleGoogle Scholar
  24. Klapec T, Borecka A. Contamination of vegetables, fruits and soil with geohelmints eggs on organic farms in Poland. Ann Agric Environ Med. 2012;19(3):421–5.PubMedGoogle Scholar
  25. Ahmad Eraky MA, Rashed S ,Nasr M, Azza ,El-Hamshary M, El-Ghannam AS. Parasitic contamination of commonly consumed fresh leafy vegetables in Benha, Egypt. J Parasitol Res. 2014; 2014: 613960. doi: 10.1155/2014/613960Google Scholar
  26. Uga S, Hoa NT, Noda S, Moji K, Cong L, Aoki Y, et al. Parasite egg contamination of vegetables from a suburban market in Hanoi, Vietnam. Nepal Med Coll J. 2009;11(2):75–8.PubMedGoogle Scholar
  27. Augustine DL. Investigations on the control of hookworm disease. X. Experiments on the length of life of infective hookworm larvae in soil. Am J Hygiene. 1922;2:177–87.Google Scholar
  28. Damen JG, Banwat EB, Egah DZ, Allanana JA. Parasitic contamination of vegetables in Jos, Nigeria. Ann Afr Med. 2007;6(3):115–8.View ArticlePubMedGoogle Scholar
  29. Mabaso MLH, Appleton CC, Hughes JC, Gouws E. The effect of soil type and climate on hookworm (Necator americanus) distribution in KwaZulu‐Natal, South Africa. Trop Med Int Health. 2003;8(8):722–7.View ArticlePubMedGoogle Scholar
  30. Daryani A, Ettehad GH, Sharif M, Ghorbani L, Ziaei H. Prevalence of intestinal parasites in vegetables consumed in Ardabil, Iran. Food Control. 2008;9:790–4.View ArticleGoogle Scholar
  31. Joghataei A, Balarak D, Mahdavi Y, Modrek JM. Prevalence of parasitic contamination of raw vegetables in Ahar, Iran. Int J Analyt Pharmaceut Biomed Sci. 2016;5(1):28–31. available online at www.ijapbs.com Accessed: Tuesday, March 22, 2016, 7:20:45 PM.Google Scholar
  32. Meerburg BG, Vermeer HM, Kijlstra A. Controlling risks of pathogen transmission by flies on organic pig farms: a review. Outlook Agriculture. 2007;36(3):193–7.View ArticleGoogle Scholar

Copyright

© The Author(s). 2017

Advertisement