Fig. 1

CRISPR/Cas9-mediated generation of the ompdc-uprt deletion mutant in the Toxoplasma gondii type I RH strain. a Diagnostic PCR of the RHΔuprt::HXGPRT clone. PCR1 is used to detect drug gene and PCR2 is used to detect uprt gene b Diagnostic PCR confirming the removal of hxgprt. PCR1 is used to detect hxgprt gene, PCR2 is used to detect uprt gene and PCR3 is used to detect internal reference 529 bp gene c Diagnostic PCR of the RHΔuprtΔompdc::HXGPRT clone. PCR1 is used to detect drug gene, PCR2 and PCR3 is used to detect ompdc gene d qRT-PCR of the RHΔuprtΔompdc::HXGPRT clone. ***P < 0.001 by Student’s t test. CRISPR clustered regularly interspaced short palindromic repeats, PCR polymerase chain reaction, HXGPRT hypoxanthine–guanine phosphoribosyltransferase, qRT-PCR quantitative reverse transcription polymerase chain reaction