Fig. 2

Deficiency of ompdc and uprt in the RH strain severely reduced parasitic proliferation in vitro. a Plaque assay comparing the growth of the RHΔku80 strain and RHΔku80ΔompdcΔuprt strain with or without the addition of 250 µmol/L uracil and 200 µmol/L UMP. b–c Number and size of the plaques. d–e Intracellular proliferation of the RHΔku80 strain and RHΔku80ΔompdcΔuprt strain with or without the addition of 250 µmol/L uracil and 200 µmol/L UMP. The number of parasites in each parasitophorous vacuole (PV) was determined at 24 h and 48 h. These results are from three independent trials. ***P < 0.001 by Student’s t test and one-way ANOVA, ns not significant. UMP uridine monophosphate, PV parasitophorous vacuole