Fig. 4

Immunization with the RHΔku80ΔompdcΔuprt mutant vaccine induced specific humoral and cellular responses. a Determination of IgG antibodies in the sera of BALB/c mice at 0, 2, 4, and 6 weeks. b Detection of antibody subtypes (IgG1 and IgG2a) in the sera of immunized mice 2 weeks after the last immunization. c–f Percentages of CD4⁺ T cells and CD8⁺ T cells subsets in immunized BALB/c mice. The results are shown as the means ± SD from three independent experiments. *P < 0.05, **P < 0.01 ***P < 0.001 by Student’s t test, ns not significant. SD standard deviation