Fig. 3

Molecular identification of the parasite in the patient from Inner Mongolia, China. A Electrophoresis of the partial18S rRNA target gene of Babesia spp. from human whole blood (Lanes 1−3); B Electrophoresis of the amplicon of a β-tubulin gene of Babesia venatorum from human whole blood (Lanes 1−2); C Electrophoresis of two amplicons of the entire 18S rRNA gene from Babesia venatorum; D Comparison analysis of the 199 base-pair nucleotide sequence alignment of babesia β-tubulin gene. E Phylogenetic analysis of the sequence from patient strain NMG01 and other members of the order Piroplasmide based on nearly entire 18S rRNA gene sequences, inferred using the use of the maximum likelihood method and bootstrap analysis of 1000 replicates to assess the reliability of the reconstructed phylogenies