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Fig. 2 | Infectious Diseases of Poverty

Fig. 2

From: Optimisation of the DNA dipstick as a rapid extraction method for Schistosoma japonicum in infected mice samples and spiked human clinical samples

Fig. 2

General workflow for DNA dipstick extraction. Modifications and optimisation steps varied depending on clinical sample used. The basic workflow involved incubation at 95 °C followed by homogenisation. After which, the DNA dipstick was dipped into the homogenate, dipped into wash buffer, then dipped into the LAMP reaction, and the LAMP reaction run. Results were viewed by colour change, and run on an agarose gel  to confirm results. LAMP: Loop Mediated Isothermal Amplification Assay

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