Fig. 3

Workflow for human stool samples seeded with cracked Shistosoma japonicum eggs. Eggs were added to negative faeces and passed through a 250 µm (pore opening size) sieve and then either sieved through a 100 µm mesh, and the filtrate retained, or on to a 40 µm sieve and the residue washed into a new tube. The resulting filtrates and residue were divided into two groups, and incubated at room temperature or at 95 °C for 30 min. DNA dipstick and LAMP were then performed. LAMP: Loop Mediated Isothermal Amplification Assay