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Fig. 4 | Infectious Diseases of Poverty

Fig. 4

From: Optimisation of the DNA dipstick as a rapid extraction method for Schistosoma japonicum in infected mice samples and spiked human clinical samples

Fig. 4

Optimisation of the DNA dipstick on spiked mice urine and sera. a DNA spiking of negative mice urine and sera. Amplification was observed after heating at 65 °C for 40 min in a water bath or 65 °C for 90 min in a thermocycler. L: Ladder, 1–3: Urine, 4–7: Sera; 8: Positive control, 9: NTC control. b Schistosoma japonicum DNA spiked mouse urine with different pre amplification conditions. 1: PK, 2: Heat, 3: Vortex, 4: PK + heat + vortex, 5: PK + heat, 6: PK + vortex, 7: Heat + vortex, 8: Positive control, 9: NTC control. PK: Proteinase K, heat: 95 °C

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Infectious Diseases of Poverty

ISSN: 2049-9957

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