Fig. 6

Optimisation of the dipstick on naturally infected human faecal samples sieved through a 40 µm or 100 µm mesh. Samples had a combination of either proteinase K (PK) added, heating (95 °C), or both prior to DNA extraction using the DNA dipstick and amplification via the LAMP assay. Amplification DNA was visualised by flocculation assay (top) or agarose gel electrophoresis (bottom). 1: 40 µm + heat; 2: 40 µm + PK + heat; 3: 40 µm + PK; 4: 40 µm; 5: 100 µm + heat; 6: 100 µm + PK + heat; 7: 100 µm + PK; 8: Positive control; 9: Negative control