Table 1 Permutations for mice samples
Faeces | Urine | Sera | |
|---|---|---|---|
DNA spiking | ✓ | ✓ | ✓ |
Heat | ✓ | ✓ | ✓ |
PK at RT | ✓ | ✓ | ✓ |
Vortex | ✓ | ✓ | ✓ |
Heat + PK | ✓ | ✓ | ✓ |
Heat + PK + vortex | ✓ | ✓ | ✓ |
PK + vortex | ✓ | ✓ | ✓ |
Heat + vortex | ✓ | ✓ | ✓ |
Heat + hand-held mortar and pestle | ✓ | ✓ | ✓ |
Lysis buffer mix | ✓ | ✓ | ✓ |
FTA card + lysis buffer | ✓ | ✓ | ✓ |
FTA card + water | ✓ | ✓ | ✓ |
FTA card + heat (50 °C) | ✓ | ✓ | ✓ |
FTA card + heat (50 °C) for 30 min | ✓ | ✓ | ✓ |
FTA card + heat (95 °C) for 30 min | ✓ | ✓ | ✓ |
Sample + dipstick | ✓ | ✓ | ✓ |
Egg spiking at RT | ✓ | ✓ | ✓ |
Egg spiking at RT for 30 min | ✓ | ✓ | ✓ |
Egg spiking at 4 °C O/N | ✓ | ✓ | ✓ |
Filtration | ✓ | × | |
Dipstick + WB + Tween 20 | × | ✓ | ✓ |
Dipstick + WB − Tween 20 | × | ✓ | ✓ |