Table 2 Permutations for human clinical samples
Faeces | Urine | Sera | |
|---|---|---|---|
1 | Heat + metal beads | Genomic DNA | Naturally infected samples |
2 | Metal beads—shake vigorously | Cracked eggs | Naturally infected samples + heat |
3 | Metal beads + PK | Genomic DNA + filter paper | – |
4 | Metal beads + PK + heat | Cracked eggs + filter paper | – |
5 | Silica beads + PK | Cracked eggs + 37 °C + 10 min | – |
6 | Silica beads + heat | Cracked eggs + 65 °C + 10 min | – |
7 | Silica beads + PK + heat | Cracked eggs + 95 °C + 10 min | – |
8 | – | Cracked eggs + 37 °C + 20 min | – |
9 | – | Cracked eggs + 65 °C + 20 min | – |
10 | – | Cracked eggs + 95 °C + 20 min | – |
11 | – | Cracked eggs + 37 °C + 30 min | – |
12 | – | Cracked eggs + 65 °C + 30 min | – |
13 | – | Cracked eggs + 95 °C + 30 min | – |
14 | – | Naturally infected samples filtered and allowed to dry O/N + lysis buffer | – |
15 | – | Naturally infected samples filtered and allowed to dry O/N + 600 µl milliQ water | – |