Table 1 Detail of the results of the first blind test showing snail species identified with the pre-existing in-lab MALDI–TOF MS database and some specimens selected to update our reference database after DNA-based identification
Morphological ID | Geographical origin (colonies) | Number of specimens tested | Good-quality MS spectra | Number of specimens used for Blind test | Snail species ID by MS | LSVa range [0–3] | Mean LSV (SD)b | Number identified with LSVs ≥ 1.7 (%) | Molecular identification (% of identity) (n = sequences) |
|---|---|---|---|---|---|---|---|---|---|
Bulinus forskalii | Central Senegal (Diourbel) | 4 | 4/4 | 4 | Bu. forskalii | [2.24–2.39] | 2.31 (0.05) | 3 (75%) | Bu. forskalii (100%) (2) |
North-Western (Senegal River Delta) | 77 | 77/77 | 77 | Bu. forskalii | [1.82–2.41] | 2.18 (0.01) | 77 (100%) | Bu. forskalii (99.35%) (8) | |
Biomphalaria pfeifferi | North-Western (Senegal River Delta) | 85 | 85/85 | 85 | Bi. pfeifferi | [1.58–2.30] | 2.09 (0.02) | 77 (90.6%) | Bi. pfeifferi (100%) (8) |
South-Eastern (Laboratory strain) | 10 | 10/10 | 10 | Bi. pfeifferi | [2.17–2.48] | 2.37 (0.03) | 10 (100%) | Bi. pfeifferi (99.84%) (4) | |
South-Eastern (Wild strain) | 6 | 6/6 | 6 | Bi. pfeifferi | [1.95–2.44] | 2.21 (0.08) | 6 (100%) | Bi. pfeifferi (99.82%) (3) | |
Total | 182 | 182/182 | 182 | 25 |