Figure 3

The Bm-ipgm specific gene silencing causes several developmental defects in the mf and the eggs. The Giemsa-stained mf from the control group (A), siRNA-free negative control group (B), and the Bm-iPGM specific siRNA-treated group (C) exhibited stretched mf sheaths. The mf from the control groups (D and E) had normal phenotypes as compared to Bm-iPGM-specific siRNA treatment that led to variable phenotypes in the mf including degenerating internal contents containing several vacuoles (F, arrows). The mf from the control groups were alive (G, H), while those from the specific siRNA-treated group were dead (I). The eggs from the control groups (J, K) were normal, while those from the iPGM specific siRNA (L) were degenerated, showing increased spaces between the eggshell and the embryo.