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Figure 6 | Infectious Diseases of Poverty

Figure 6

From: In vitro gene silencing of independent phosphoglycerate mutase (iPGM) in the filarial parasite Brugia malayi

Figure 6

Effect of siRNA on Bm-ipgm gene transcription in the L3 stage and the production of adult worms. (A) siRNA treatment of infective larvae (L3) resulted in a significant loss of the Bm-iPGM transcript level after 24 and 48 hours of incubation. (B) The number of recovered male and female worms from the control, negative control, and Bm-ipgm-specific treated L3 implanted jirds. The Bm-ipgm-specific siRNA-treated L3 exhibited 44.36% reduction in the adult worm establishment in the peritoneal cavity of naive male jird, which was significant (p <0.01) over control. (C) The percentage of sterile female worms recovered from control, negative control, and Bm-ipgm-specific treated L3 implanted jirds. A significant number of female worms recovered from the siRNA-treated group displayed defective embryogenesis. The recovered females were teased in a drop of PBS on glass slide and the condition of intrauterine contents was analyzed microscopically (Nikon). The percentage of sterile female worms was calculated with respect to the total female worms recovered and analyzed. The criterion for statistical significance between the results was carried out by ANNOVA and was expressed in the form of p value that was considered significant and marked as p <0.05 *, p <0.01 as highly significant **, and p <0.001 *** as very highly significant. The data represent the mean ± SE of percent reduction in mf counts from the two independent experiments.

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