In vitro gene silencing of independent phosphoglycerate mutase (iPGM) in the filarial parasite Brugia malayi

Table 4 The effect of Bm-iPGM siRNA treatment on the morphology of infective larvae (L3)

Infective Larvae condition	Control		Negative control		Bm-iPGM specific siRNA
Infective Larvae condition	24 h	48 h	24 h	48 h	24 h	48 h
*Active (%)*	82.5 ± 4.9	58.5 ± 12.0	82.5 ± 7.7	57.5 ± 9.1	49 ± 2.12	23.5 ± 9.1
*Sluggish (%)*	10.0 ± 2.8	17.5 ± 3.5	12.5 ± 3.5	17.0 ± 5.6	37.0 ± 2.12	50.5 ± 3.5
**Dead (%)***	7.5 ± 2.2	24.0 ± 8.4	10.0 ± 2.8	25.5 ± 3.5	14.0 ± 4.24	26.0 ± 12.7

*: These were the non-motile L3 where no reversal in motility was noticed even after transfer to siRNA-free medium.

ISSN: 2049-9957