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Table 4 The effect of Bm-iPGM siRNA treatment on the morphology of infective larvae (L3)

From: In vitro gene silencing of independent phosphoglycerate mutase (iPGM) in the filarial parasite Brugia malayi

Infective Larvae condition Control Negative control Bm-iPGM specific siRNA
24 h 48 h 24 h 48 h 24 h 48 h
Active (%) 82.5 ± 4.9 58.5 ± 12.0 82.5 ± 7.7 57.5 ± 9.1 49 ± 2.12 23.5 ± 9.1
Sluggish (%) 10.0 ± 2.8 17.5 ± 3.5 12.5 ± 3.5 17.0 ± 5.6 37.0 ± 2.12 50.5 ± 3.5
Dead* (%) 7.5 ± 2.2 24.0 ± 8.4 10.0 ± 2.8 25.5 ± 3.5 14.0 ± 4.24 26.0 ± 12.7
  1. *: These were the non-motile L3 where no reversal in motility was noticed even after transfer to siRNA-free medium.