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Figure 1 | Infectious Diseases of Poverty

Figure 1

From: Molecular evidence on the occurrence of co-infection with Pichia guilliermondii and Wuchereria bancrofti in two filarial endemic districts of India

Figure 1

Molecular identification and phylogeny of P. guilliermondii isolated from filarial patients. A. Agarose gel showing PCR amplicon of 18S rDNA of W. bancrofti, Wolbachial 16S rDNA, and Wolbachia surface protein. M: DNA ladder (100 bp to 3 kb), L1: filarial 28S rDNA specific amplicon, L2: Amplified 16S rDNA of Wolbachia, L3: wsp-int specific amplicon. B. Agarose gel representing the PCR amplicon of D1/D2 region of 26S rDNA of P. guilliermondii. C. Agarose gel showing PCR amplicon of P. guilliermondii DNA amplified with RPS0 gene specific primers. D. Maximum Parsimony analysis of the phylogenetic relationship between P. guilliermondii and some common yeast species. The numerals given on the branches of the tree indicate % bootstrap value (BV). In the tree, BV values less than 50 were considered low. Figure A, B, and C are the representative of experiments carried out in triplicates and repeated at least five times.

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