Molecular evidence on the occurrence of co-infection with Pichia guilliermondii and Wuchereria bancrofti in two filarial endemic districts of India

Table 1 Oligonucleotide primers used for the PCR-based identification of the fungal isolate and Wolbachia

Gene	Oligonucleotide sequence	Source/Reference
Filaria-specific 28S rRNA (BD1A)	Forward: 5′ATGAAAGGCGTTGATATATAG3′	Gayen et al., [22]
Filaria-specific 28S rRNA (BD1A)	Reverse: 5′GCAAGCCATGCAAGCGTTGAG3′	Gayen et al., [22]
Wolbachia 16S rRNA-specific	Forward (FIL-5): 5′ TGAGGAAGATAATGACGG3′	Smith and Rajan, 2000 [25]
Wolbachia 16S rRNA-specific	Reverse (FIL-6): 5′CCTCTATCCTCTTTCAACC3′	Smith and Rajan, 2000 [25]
WSP int	Forward: 5′TAGCTTACTACATTCGCTTGCA3′	Bazzocchi et al., 2000 [26]
WSP int	Reverse: 5′CCAACTAGTGCCTATAAAGAAC3′	Bazzocchi et al., 2000 [26]
26 s rDNA	Forward (NL1): 5′GCATATCAATAAGCGGAGGAAAAG3′	O’Donnell, 1993 [27]
26 s rDNA	Reverse (NL4): 5′GGTCCGTGTTTCAAGACGG3′	O’Donnell, 1993 [27]
RPS0	Forward: 5′CTTGGGTTCCAAGAACGTGATT3′	Martinez et al., [24]
RPS0	Reverse: 5′CTTCAGCATTCCTCAGCCTTGGA3′	Martinez et al., [24]

ISSN: 2049-9957