Fig. 4
From: Prediction of the next highly pathogenic avian influenza pandemic that can cause illness in humans
In vitro coupled transcription/translation assay with representative HA 5′-UTR sequences. Seven 40 bp 5′-UTR sequences (including two nucleotide substitution variants) were introduced between the T7 promoter and the Luciferase template, and then the in vitro transcription/translation coupled reaction was performed using the TNT® T7 Coupled Reticulocyte Lysate System. Luciferase activity of the control construct (T7 promoter – Luciferase fusion) was normalized at 100 relative units