Table 1 Target sequences used for detecting schistosomiasis in humans
From: Nucleic acid detection in the diagnosis and prevention of schistosomiasis
Target sequences | Method [Ref.] | Detection limit | Sensitivity % | Specificity % |
|---|---|---|---|---|
Repetitive sequences | ||||
SjCHGCS19 | Nested PCR [41] | 2.02 copies | 97.67 | 96.07 |
SjR2 (AF412221) | PCR [24] | 0.8 pg | 60 | 100 |
Real-time PCR [38] | 44.7 copies | — | — | |
LAMP [7] | 0.08 fg | 96.7 | 100 | |
Nested PCR [40] | — | — | — | |
SM1-7 (M61098) | 1 fg | 96.7 | 88 | |
Real-time PCR [26] | 0.02 egg | — | — | |
LAMP [10] | 0.1 fg | — | — | |
Touchdown PCR [43] | 10 fg | — | — | |
Dra I (DQ157698) | PCR [44] | 10 fg | — | — |
Real-time PCR [23] | 0.5 EPG | — | — | |
LAMP [10] | 0.1 fg | — | — | |
Ribosomal DNA | ||||
18 s rDNA | Nested PCR [46] | 10 fg | — | — |
PCR [47] | 40 pg/ul | — | — | |
Real-time PCR [25] | 0.01 pg | — | — | |
28 s rDNA | Multiplex PCR [48] | — | 94.4 | 99.9 |
PCR [49] | — | — | — | |
LAMP [9] | 100 fg | — | — | |
SSU-rDNA (X53047) | Real-time PCR [50] | 10 fg | — | — |
Mitochondrial DNA | ||||
NADH I | Real-time PCR [51] | 1 EPG | — | — |
NADH-3 | PCR [55] | 1 pg | — | — |
COX I | PCR [56] | 0.01 pg | — | — |