Table 1 Primers used in the present study to determine the genetic structure of A. cantonensis in P.R. China
From: The genetic variation of Angiostrongylus cantonensis in the People’s Republic of China
Gene | Primer | Primer sequence | Annealing temperature |
|---|---|---|---|
nad1 | ND1_F | 5′-GATTTAGTTATTCTTGTTG-3’ | 48 °C |
ND1_R | 5′-CCAACAAAAACACATCTAAC-3’ | ||
cox1 | COI_F1 | 5′-GGTGATTATAATGTTTAATG-3’ | 53 °C |
COI_R1 | 5′-CGTAGGAACCGCAATAAC-3’ | ||
COI_F2 | 5′-TATGGTTTATGCTATTTTAAG-3’ | 53 °C | |
COI_R2 | 5′-GGCACTACACAACGATTATC-3’ | ||
ITS | ITS_F | 5′-GTAGGTGAACCTGCGGAAGGATCATT-3’ | 55 °C |
ITS_S | 5′-TTAGTTTCTTTTCCTCCGCT-3’ |