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Table 1 UCP-LF CAA assay formats for urine analysis

From: Utilizing the ultrasensitive Schistosoma up-converting phosphor lateral flow circulating anodic antigen (UCP-LF CAA) assay for sample pooling-strategies

Assay
format
Analyzed
volume (μl)
TCA extraction solution
volume (μl) / % (w/v)
Amicon (centrifugal)
concentration device
QC threshold 1
singlet test (pg/ml)
UCAA10 10 μl 10 μl / 4% none 10
UCAA250 250 μl 250 μl / 4% 0.5 ml Filter Tube 1
UCAA2000 2 000 μl 2 000 μl / 4% 4 ml Filter Tube 0.1
UCAA14000 14 000 μl 1 000 μl / 30%2 15 ml Filter Tube3 0.03
UCAA40000 40 000 μl 10 000 μl / 10%2 50 ml Cell3 0.01
  1. 1Quality control threshold (CAA concentration) for single test with wet reagents [20]
  2. 2Utilizing relevantly higher TCA concentration than the 4% (w/v) applied in the standard formats
  3. 3The 15 ml filter tube and 50 ml cell concentration is a two-step procedure requiring an additional 0.5 ml filter tube to concentrate down to 20 μl