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Table 3 Pooling strategy maintaining ultimate (UCAA2000) sensitivity

From: Utilizing the ultrasensitive Schistosoma up-converting phosphor lateral flow circulating anodic antigen (UCP-LF CAA) assay for sample pooling-strategies

  Number of pools Number of positive individuals
Individuals pool size = 35 Prevalence 5% Prevalence 1% Prevalence 0.2%
35 1 2 1 1
70 2 4 1 1
140 4 7 2 1
280 8 14 3 1
560 16 28 6 2
1 120 32 56 12 3
  Number of pools Number of positive pools
Individuals pool size = 35 Prevalence 5% Prevalence 1% Prevalence 0.2%
35 1 1 (100%) 1 (100%) 1 (100%)
70 2 2 (100%) 1 (50%) 1 (50%)
140 4 4 (100%) 2 (50%) 1 (25%)
280 8 8 (100%) 3 (37.5%) 1 (12.5%)
560 16 16 (100%) 6 (37.5%) 2 (12.5%)
1 120 32 32 (100%) 12 (37.5%) 3 (9.4%)
  Number of pools Number of individuals not requiring an individual test
Individuals pool size = 35 Prevalence 5% Prevalence 1% Prevalence 0.2%
35 1 0 0 0
70 2 0 35 35
140 4 0 70 105
280 8 0 175 245
560 16 0 350 490
1 120 32 0 700 1 015
  1. Relation between prevalence and number of negative pools and samples not requiring an individual test results. At low prevalence the number of negative pools increases, hence less individuals require an individual result implying relevant reduction of the number of tests. Calculations are based on equal distribution of the ‘CAA-positive’ individuals, the least favourable condition leading to the highest number of individual tests. In real pooling applications, the number of negative pools expectedly will be higher than shown the above example due to non-random distribution of the ‘CAA-positive’ individuals