Stage
|
MEC (μg/ml) producing
|
Time of onset of vesiculation
|
Vesicle numberb
|
---|
Stimulation
|
Contraction
|
Vesiculationa
|
---|
S. mansoni
|
Reference [26]
|
Day 0
|
0.005
|
0.010
|
0.1
|
2–4 h
|
Few
|
Day 3
|
0.010
|
0.010
|
1.0
|
> 4 h
|
Few
|
Day 7
|
0.010
|
0.010
|
1.0
|
> 4 h
|
Few
|
Day 14
|
0.010
|
0.010
|
0.1
|
30 min
|
Many
|
Day 28
|
0.005
|
0.010
|
0.1
|
30 min
|
Many
|
Day 35
|
0.005
|
0.010
|
0.1
|
5–15 min
|
Very many
|
Day 42
|
0.005
|
0.010
|
0.1
|
5–15 min
|
Very many
|
S. japonicum
|
Reference [41]
|
Day 0
|
0.01
|
0.05
|
0.5
|
3–4 h
|
Few
|
Day 3
|
0.01
|
0.05
|
1.0
|
24 h
|
Very few
|
Day 7
|
0.01
|
0.05
|
1.0
|
24 h
|
Very few
|
Day 14
|
0.01
|
0.05
|
1.0
|
2 h
|
Many
|
Day 21
|
0.01
|
0.05
|
0.1
|
2 h
|
Many
|
Day 28
|
0.005
|
0.05
|
0.1
|
2 h
|
Many
|
Day 35
|
0.005
|
0.05
|
0.1
|
15 min
|
Very many
|
Day 42
|
0.005
|
0.05
|
0.1
|
15 min
|
Very many
|
- aObserved by light microscopy
- bFew ≤ 5 vesicle per parasite, many = 5–20 vesicles per parasite, and very many ≥20 vesicles per parasite