Fig. 1

Depiction of a positive real-time RT-PCR result for CCHF virus detection. Amplification plots generated during RT-PCR for virus detection are shown. The number of PCR cycles is given on X-axis and the Delta Rn values are plotted on Y-axis. The green line is called the threshold baseline (A). Delta Rn represents the change in the fluorescent cycle. The fractional cycle number at which that sample crosses the threshold is called the cycle of threshold (=Ct). As shown in the graph the fluorescent peak of positive samples (=PS) crosses the threshold baseline while the negative samples (=NS) yield very low fluorescent signals below the threshold baseline