Molecular detection of Anaplasma infections in ixodid ticks from the Qinghai-Tibet Plateau

Table 1 Primers used for PCR for the identification of tick species and detection and of Anaplasma spp. in the ticks from Qinghai

Target species	Target gene	Primer(5′ → 3′)	Annealing temperature (°C)	No. of cycles	Expected size (bp)	References
Anaplasma spp.	16S rRNA	EE1: TCCTGGCTCAGAACGAACGCTGGCGGC EE2: AGTCACTGACCCAACCTTAAATGGCTG	55	35	1400	[37]
A. bovis	16S rRNA	AB1f: CTCGTAGCTTGCTATGAGAAC AB1r: TCTCCCGGACTCCAGTCTG	55	35	551	[26]
A. phagocytophilum	16S rRNA	SSAP2f: GCTGAATGTGGGGATAATTTAT SSAP2r: ATGGCTGCTTCCTTTCGGTTA	55	35	641	[26]
A. marginale	msp4	Amargmsp4F: CTGAAGGGGGAGTAATGGG Amargmsp4R: GGTAATAGCTGCCAGAGATTC	60	30	344	[38]
A. ovis	msp4	MSP43: CCGGATCCTTAGCTGAACAGAATCTTGC MSP45: GGGAGCTCCTATGAATTACAGAGAATTGTTTAC	60	35	869	[39]
A. capra	gltA	gltAouterF: GCGATTTTAGAGTGYGGAGATTG gltAouterR: TACAATACCGGAGTAAAAGTCA	55	35	1031	[1]
		gltAinnerF: GCGATTTTAGAGTGYGGAGATTG gltAinnerR: GCGATTTTAGAGTGYGGAGATTG	60	35	594
	16S rRNA	Forward: GCAAGTCGAACGGACCAAATCTGT Reverse: CCACGATTACTAGCGATTCCGACTTC	58	35	1261	[35]
	groEL	Forward: TGAAGAGCATCAAACCCGAAG Reverse: CTGCTCGTGATGCTATCGG	55	35	874	[35]
Tick	16S rRNA	16SrRNA-F: CTGCTCAATGATTTTTTAAATTGCTGTGG 16SrRNA-R: CCGGTCTGAACTCAGATCAAGT	55	35	450	Designed for this study

ISSN: 2049-9957