Table 1 Primers used to amplify the mitochondrial 16S rRNA, COI genes and the nuclear ribosomal 28S rRNA gene
Gene target | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Expected length (bp) |
|---|---|---|---|
16S rRNA | GGTTTTGAATGGCCGCAGTA | TCTCCGGTTTGAACTCAGATCA | 491 |
28S rRNA | GCGAGTCGTGTTGCTTGATAGTGCAG | TTGGTCCGTGTTTCAAGACGGG | 678 |
COI | TGTAGAAAGAGGAGCGGGAA | TCCGGTTTCCATGGCAATAA | 439 |