A multiplex PCR for differential detection of Echinococcus granulosus sensu stricto, Echinococcus multilocularis and Echinococcus canadensis in China

Table 3 Primers designed for detection of Echinococcus granulosus s. s., E. multilocularis and E. canadensis in the study

Targeted species	Primer name	Sequences (5′ → 3′)	Expected product length	Final concentration (nmol/L)
E. granulosus s. s.	g/f	GTCTGTGTTTCTTACCATTG	811	200
E. granulosus s. s.	g/r	GACCCGTACAAACATATATCAAC	811	200
E. multilocularis	m/f	TTGTTCTTTGTGTTACTGTAGG	457	600
E. multilocularis	m/r	CTATACAGACATTGATTACCATAA	457	600
E. canadensis	c/f	GTAAGTCTAAGTTGGTTATTATTCAC	315	200
E. canadensis	c/r	CTTATTAAACAACACAAAAATACTAAATG	315	200

ISSN: 2049-9957