Table 2 Primers for amplification of mitochondrial genome fragments
Amplication fragments | Primer name | Sequence (5′ → 3′) | Annealing temperature (°C) |
|---|---|---|---|
F5: COI + tRNA + COII | 5-F2637 | AGCAGGWTTTRTYCAYTGAT | 45 |
5-R3590 | CTCCTAAAGCWGGKAYTGTT | ||
F7: ATP6 + COIII | 7-F4076 | ATTTTCYGTATTYGACCCYTC | 47 |
7-R4929 | TCTCGWGAWACATCTCGTCAT | ||
F8: ATP6 + COIII | 8-F4518 | CGACCWGGAACWTTAGCWGT | 47 |
8-R5523 | TAYCCTCCTCATCARTAAAT | ||
F19: ND1 | 19-F11982 | AAAGCAAAWCCYCCTCTTC | 47 |
19-R12558 | ATATTCAAATTCGTAARGG | ||
F21: lrRNA | 21-F12834 | TTACRCCGGTTTGAACTCAG | 47 |
21-R13356 | WTAAAGTCTAACCTGCCCAC |