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Table 2 Primers for amplification of mitochondrial genome fragments

From: Phylogeny of certain members of Hyrcanus group (Diptera: Culicidae) in China based on mitochondrial genome fragments

Amplication fragments Primer name Sequence (5′ → 3′) Annealing temperature (°C)
F5: COI + tRNA + COII 5-F2637 AGCAGGWTTTRTYCAYTGAT 45
5-R3590 CTCCTAAAGCWGGKAYTGTT
F7: ATP6 + COIII 7-F4076 ATTTTCYGTATTYGACCCYTC 47
7-R4929 TCTCGWGAWACATCTCGTCAT
F8: ATP6 + COIII 8-F4518 CGACCWGGAACWTTAGCWGT 47
8-R5523 TAYCCTCCTCATCARTAAAT
F19: ND1 19-F11982 AAAGCAAAWCCYCCTCTTC 47
19-R12558 ATATTCAAATTCGTAARGG
F21: lrRNA 21-F12834 TTACRCCGGTTTGAACTCAG 47
21-R13356 WTAAAGTCTAACCTGCCCAC