|
POC-CCA†
|
Reference test
|
Sensitivity, % (95% CI)
|
Specificity, % (95% CI)
|
PPV, % (95% CI)
|
NPV, % (95% CI)
|
Accuracy, % (95% CI)
|
Kappa index (95% CI)
|
|---|
|
+
|
−
|
|---|
| |
KK
|
|
+
|
32
|
19
|
29.6 (21.2–39.2)
|
93.8 (90.4–96.2)
|
62.8 (48.1–75.9)
|
79.0 (74.4–83.0)
|
77.0 (72.6–81.0)
|
0.282 (0.179–0.385)
|
|
−
|
76
|
285
|
| |
F_ddPCR
|
|
+
|
44
|
7
|
14.3 (10.6–18.8)
|
93.3 (86.8–97.3)
|
86.3 (73.7–94.3)
|
27.2 (22.6–32.1)
|
34.5 (29.9–39.3)
|
0.043 (0.007–0.078)
|
|
−
|
263
|
98
|
| |
SR_ddPCR
|
|
+
|
41
|
10
|
14.8 (10.8–19.5)
|
92.6 (86.8–96.4)
|
80.4 (66.9–90.2)
|
34.6 (29.7–39.8)
|
40.1 (35.5–45.2)
|
0.052 (0.008–0.095)
|
|
−
|
236
|
125
|
| |
U_ddPCR
|
|
+
|
31
|
20
|
15.8 (11.0–21.7)
|
90.7 (86.1–94.3)
|
60.8 (46.1–74.2)
|
54.3 (49.0–59.5)
|
55.1 (50.2–60.0)
|
0.068 (0.001–0.134)
|
|
−
|
165
|
196
|
| |
SL_ddPCR
|
|
+
|
29
|
22
|
27.6 (19.3–37.2)
|
92.8 (89.4–95.5)
|
56.9 (42.3–71.0)
|
79.0 (74.4–83.0)
|
76.2 (71.8–80.3)
|
0.246 (0.142–0.350)
|
|
−
|
76
|
285
|
| |
Sj23-LHD-ELISA#
|
|
+
|
23
|
28
|
22.3 (14.7–31.6)
|
91.0 (89.4–95.5)
|
45.1 (31.1–59.7)
|
77.8 (73.2–82.0)
|
73.8 (69.3–78.0)
|
0.160 (0.058–0.261)
|
|
−
|
80
|
281
|
| |
SjSAP4-ELISA#
|
|
+
|
41
|
10
|
15.6 (11.4–20.6)
|
93.3 (88.0–96.7)
|
80.4 (66.9–90.2)
|
38.5 (33.5–43.7)
|
43.7 (38.8–48.6)
|
0.068 (0.021–0.114)
|
|
−
|
222
|
139
|
- CI Confidence interval, ddPCR Droplet digital PCR, The ddPCRs performed on faeces, serum, urine and saliva were designated as F_ddPCR, SR_ddPCR, U_ddPCR and SL_ddPCR, respectively. ELISA Enzyme-linked immunosorbent assay, KK Kato-Katz, POC-CCA Point-of-care circulating cathodic antigen, NPV Negative predictive value, PPV Positive predictive value.
- †R cut-off value: 0.1344
- #Cut-off values for ELISA assays: Sj23-LHD-ELISA, 0.2185; SjSAP4-ELISA, 0.1832 [25]
