Table 1 Primer sequences for the SOE PCR assay
Gene/region | Primer | Sequence (5′–3′) |
|---|---|---|
ropB | rpoB_For | GTACGGTCGGCGAGCTGATCCA |
rpoB_Rev | CACCGGGTGCACGTCGCGGACCTCCAGCCCGGCACGCTCACGTGACAGAC | |
embB | embB_For | GGAGGTCCGCGACGTGCACCCGGTGATATTCGGCTTCCTGCTC |
embB_Rev | ACGGAAGGGATCCTCCGGGCTGCCGAACCAGCGGAAATAGTTGGA | |
katG | katG_For | CGGCAGCCCGGAGGATCCCTTCCGTATGGCACCGGAACCGGTAA |
katG_Rev | ACGCAAGCGCCAGCAGGGCTCTTCGTCAGCTCCCACTCGTAGCCGTACA | |
inhA promoter | inhA_For | GACGAAGAGCCCTGCTGGCGCTTGCGTAACCCCAGTGCGAAAGTTCCCG |
inhA_Rev | GGACTGAACGGGATACGAATGG | |
| Â | TBseq (Sequencing primer) | ACCAGATCCGGGTCGGCATG |