Infectious Diseases of Poverty

Table 4 Sensitivity, specificity, PPV, NPV, and agreement of the SOE PCR assay compared with phenotypic DST among M. tuberculosis isolates

From: Establishment and evaluation of an overlap extension polymerase chain reaction technique for rapid and efficient detection of drug-resistance in Mycobacterium tuberculosis

Drugs	DST	SOE PCR
Drugs	DST	R	S	Sensitivity (%)	Specificity (%)	PPV (%)	NPV (%)	Concordance (%)	Kappa value
RFP	R	58	0	100.00	88.00	90.63	100.00	94.44	0.89
RFP	S	6	44	100.00	88.00	90.63	100.00	94.44	0.89
INH	R	53	3	94.64	94.23	94.64	94.23	94.44	0.89
INH	S	3	49	94.64	94.23	94.64	94.23	94.44	0.89
EMB	R	20	9	68.97	79.75	55.56	87.50	76.85	0.45
EMB	S	16	63	68.97	79.75	55.56	87.50	76.85	0.45

S susceptible; R resistant; PPV positive predictive value; NPV negative predictive value; DST drug susceptibility testing; RFP rifampicin; INH isoniazid; EMB ethambutol

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ISSN: 2049-9957

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