Table 2 Prevalence of point mutations during different time periods

pvdhfr

n = 13

n = 30

n = 144

n = 187

C49R

1 (7.7)

0 (0)

1 (0.7)

2 (1.1)

F57I/L

11 (84.6)

18 (60.0)

47 (32.6)***

76 (40.6)

S58R

12 (92.3)

21 (70.0)

73 (50.7)**

106 (56.7)

T61M

11 (84.6)

17 (56. 7)

47 (32.6)***

75 (40.1)

H99S/R

2 (15.4)

10 (33.3)

91 (63.2)***

103 (56.6)

S117T/N

12 (92.3)

19 (63.3)

71 (49.3)**

102 (56.0)

I173F

0 (0)

1 (3.3)

0 (0)

1 (0.6)

pvdhps

n = 13

n = 30

n = 184

n = 227

A372S

0 (0)

0 (0)

1 (0.5)

1 (0.4)

S382A/C

2 (15.4)

3 (10.0)

14 (7.6)

19 (8.4)

A383G

12 (92.3)

28 (93.3)

117 (63.6)***

157 (69.2)

K512E

0 (0)

1 (3.3)

11 (6.0)

12 (5.3)

A553G

8 (61.5)

16 (53.3)

46 (25.0)***

70 (30.8)

E571Q

0 (0)

0 (0)

9 (4.9)

9 (4.0)

G626A

0 (0)

0 (0)

4 (2.2)

4 (1.8)

A633S

0 (0)

0 (0)

3 (1.6)

3 (1.3)

D639E

0 (0)

0 (0)

9 (4. 9)

9 (4.0)

S640G

0 (0)

0 (0)

9 (4. 9)

9 (4.0)

A647S

0 (0)

1 (3.3)

32 (17.4)

33 (15.5)

pvmdr1

n = 13

n = 30

n = 189

n = 232

T958M

13 (100)

30 (100)

189 (100)

232 (100)

Y976F

4 (30.8)

1 (3.3)

0 (0)

5 (2.2)

K997R

0 (0)

1 (3.3)

7 (3.7)

8 (3.5)

F1076L

8 (61.5)

17 (56.7)

143 (75.7)

168 (72.4)

pvcrt-o

n = 12

n = 29

n = 158

n = 199

T2I

0 (0)

0 (0)

1 (0.6)

1 (0.5)

K10 insertion

7 (58.3)

11 (37.9)

48 (30.4)

66 (33.2)

R19C

1 (8.3)

0 (0)

0 (0)

1 (0.5)

N57H

0 (0)

1 (3. 5)

0 (0)

1 (0.5)