Table 2 Sequence of primers and probes for the real-time PCR for CL and VL identification
From: Establish an allele-specific real-time PCR for Leishmania species identification
Diseases | Target gene | Species | Primer and probe | Sequences | Amplicon size (bp) | GeneBank accession no. |
|---|---|---|---|---|---|---|
CL | SPDSYN |  | CL-SPD-F | 5ʹ-AGATCATTGCGTACTTGAC-3ʹ | 202 |  |
|  | CL-SPD-R | 5ʹ-TCATCGACACAACAGACC-3ʹ | ||||
L. major | P-SPD-LM | 5ʹ-VIC-TCAGGAACCCCATCATCT-MGB-NFQ-3ʹ |  | KC159695 | ||
L. tropica | P-SPD-LT | 5ʹ-FAM-TCAGGAAGCCCATCATCT- MGB-NFQ-3ʹ |  | KM086079 | ||
L. donovani/infantum | P-SPD-LDI | 5ʹ-Texas Red -TCAGGAATCCCATCATCA- MGB-NFQ-3ʹ |  | AF298195 | ||
VL | HGPRT |  | VL-HGPRT-F2 | 5ʹ-AGAAGATTGCAGAGGACT-3ʹ | 145 |  |
|  | VL-HGPRT-R1 | 5ʹ-AACTCCACCTTCACCGGGACA-3ʹ | ||||
L. donovani | P-HGPRT-LD | 5ʹ-FAM- ACAGAAGTTTTAAGTTGACGACC-MGB-NFQ-3ʹ |  | AB709805 | ||
L. infantum | P-HGPRT-LI | 5ʹ-VIC- ACAGAAGCTTTAAATTGACGACC- MGB-NFQ-3ʹ |  | XM_003392399 |