Table 4 The diagnostic performance of reference laboratory tests on dried blood spot for diagnosis of human African trypanosomiasis
Reference laboratory test | % Sensitivity (n/N, 95% CI) | % Specificity (n/N, 95% CI) | % PPV (n/N, 95% CI) | % NPV (n/N, 95% CI) | % Accuracy (n/N, 95% CI) |
|---|---|---|---|---|---|
Trypanolysis (in parallel) | 85.3 (29/34, 68.9–95.0) | 92.9 (39/42*, 80.5–98.5) | 90.6 (29/32, 75.0–98.0) | 88.6 (39/44, 75.4–96.2) | 89.5 (68/76, 80.3–95.3) |
TL LiTat 1.3 | 79.4 (27/34, 62.1–91.3) | 92.9 (39/42*, 80.5–98.5) | 90.0 (27/30, 73.5–97.9) | 84.8 (39/46, 71.1–93.7) | 86.8 (66/76, 77.1–93.5) |
TL LiTat 1.5 | 55.9 (19/34, 37.9–72.8) | 92.9 (39/42*, 80.5–98.5) | 86.4 (19/22, 65.1–97.1) | 72.2 (39/54, 58.4–83.8) | 76.3 (58/76, 65.2–85.3) |
ELISA/T.b. gambiense | 67.6 (23/34, 49.5–82.6) | 95.3 (41/43, 84.2–99.4) | 92.0 (23/25, 74.0–99.0) | 78.8 (41/52, 65.3–88.9) | 83.1 (64/77, 72.9–90.7) |
m18S qPCR | 44.1 (15/34, 27.2–62.1) | 97.7 (42/43, 87.7–99.9) | 93.8 (15/16, 69.8–99.8) | 68.9 (42/61, 55.7–80.1) | 74.0 (57/77, 62.8–83.4) |
LAMP | 36.4 (12/33*, 20.4–54.9) | 93.0 (40/43, 80.9–98.5) | 80.0 (12/15, 51.9–95.7) | 65.6 (40/61, 52.3–77.3) | 68.4 (52/76, 56.7–78.6) |