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Fig. 4 | Infectious Diseases of Poverty

Fig. 4

From: Mosquito densovirus significantly reduces the vector susceptibility to dengue virus serotype 2 in Aedes albopictus mosquitoes (Diptera: Culicidae)

Fig. 4

Immunofluorescence imaging of C6/36 cells monoinfected or superinfected with AaeDV and DENV-2. A C6/36 cells were subjected to AaeDV infection and then superinfected with DENV-2 for 12, 24, 48, 72, 96, and 120 h. AaeDV and DENV-2 monoinfection treatments were used as controls. The cells were fixed and permeabilized with an acetone/absolute alcohol mixture at the respective time points post infection and immunostained for DENV (green, AF488) and AaeDV (red, AF594). Images were captured under a microscope. DAPI was used to stain the nucleus (blue). B The top-right insets show a zoomed image of cells harboring both viruses. C A bar chart presents the percentage of C6/36 cells infected with AaeDV and DENV in monoinfection compared to superinfection. The given values are the mean ± SD of three independent replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001

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