Differential diagnosis of coronavirus disease 2019 from community acquired - pneumonia by CT scan and follow-up

Objective: Coronavirus disease 2019 (COVID-19) is currently the most serious infectious disease in the world. An accurate diagnosis of this disease in the clinic is very important. This study aims to improve the differential ability of computed tomography (CT) to diagnose COVID-19 and other community-acquired pneumonias (CAPs) and evaluate the short-term prognosis of these patients. Methods: The clinical and imaging data of 165 COVID-19 and 118 CAP patients diagnosed in seven hospitals in Anhui Province, China from January 21 to February 28, 2020 were retrospectively analysed. The CT manifestations of the two groups were recorded and compared. A correlation analysis was used to examine the relationship between COVID-19 and age, size of lung lesions, number of involved lobes, and CT �ndings of patients. The factors that were helpful in diagnosing the two groups of patients were identi�ed based on speci�city and sensitivity. Results : The typical CT �ndings of COVID-19 are simple ground-glass opacities (GGO), GGO with consolidation or grid-like changes. The sensitivity and speci�city of the combination of age, white blood cell count, and ground-glass opacity in the diagnosis of COVID-19 were 92.7% and 66.1%, respectively. Pulmonary consolidation, �brous cords, and bronchial wall thickening were used as indicators to exclude COVID-19. The sensitivity and speci�city of the combination of these �ndings were 78.0% and 63.6%, respectively. The follow-up results showed that 67.8% (112/165) of COVID-19 patients had abnormal changes in their lung parameters, and the severity of the pulmonary sequelae of patients over 60 years of age worsened with age. Conclusions : Age, white blood cell count and ground-glass opacity have high accuracy in the early diagnosis of COVID-19 and the differential diagnosis from CAP. Patients aged over 60 years with COVID-19 have a poor prognosis. This result provides certain signi�cant guidance for the diagnosis and treatment of new coronavirus pneumonia.

The gold standard for diagnosing COVID-19 is a positive result for the new coronavirus nucleic acid test [8,9], but due to delays in obtaining test results or "false-negatives" in some reports, some patients cannot be diagnosed and treated in time.COVID-19 has certain imaging characteristics in the lungs.Chest computed tomography (CT) examination is an important method for the clinical diagnosis of COVID-19 [10].The early detection of lesions is very important for early isolation and treatment and preventing the spread of the disease.Previous studies have mostly focused on the imaging manifestations and changes in COVID-19 [11][12][13].The disease needs to be distinguished from many diseases by imaging, especially community-acquired pneumonia (CAP).However, comparative studies based on imaging in this area remain lacking.
The purpose of this study was to compare the clinical features and CT imaging manifestations of COVID-19 with those of community-acquired pneumonia, explore the value of CT in the diagnosis and differential diagnosis of COVID-19 and evaluate the short-term prognosis of patients with these two types of pneumonia.

CT examination
CT scans were performed for all patients with 64-slice multidetector row CT scanners.All images were displayed and stored in the picture archiving and communication systems (PACS) system.Information regarding the CT systems in the seven hospitals is provided in Supplementary Table 1. .

CT image evaluation
The CT images of the patients in the COVID-19 and CAP groups were acquired by three experienced radiologists (who have been working in radiography for over 5 years).A double-blind method was used, and the images were read independently.The radiologists carefully observed and recorded the location, shape, number, size, edge pattern and extent of the lesion.If the diagnosis was inconsistent, a consensus was reached by the three experts.

Statistical analysis
The measurement data are expressed as the means ± SDs, and the comparison of the groups was conducted by an independent-samples t-test; the count data are expressed as percentages (%), and the comparisons of the groups were conducted by the χ 2 test.A correlation analysis was used to examine the relationship between COVID-19 and the age, size of the lung lesions, number of involved lobes, and CT ndings of the patients.The statistical software SPSS version 22.0 (IBM Corporation, Armonk, NY, USA) was used for the statistical analysis.

Study population characteristics
The baseline data and clinical manifestations of the two groups are shown in Table 1.The differences in age, white blood cell (WBC) count, lymphocyte proportion, and presence or absence of underlying disease in the lungs between the two groups of patients were signi cant (χ 2 = 8.03, P < 0.001; χ 2 = 5.58, P < 0.001; t = 4.62, P = 0.003; χ 2 = 21.72,P < 0.001).

CT imaging
The chest CT ndings of the patients who had COVID-19 or CAP for 3-7 days are shown in Table 2. Ground-glass opacities (GGOs) on chest CT images were found in 140 patients (84.8%) in COVID-19 group.The typical early lesions in COVID-19 group appeared as single or multiple small round lesions.In this group, 53 patients (32.1%) had small round lesions, and 87 patients (52.7%) had ground-glass-like density shadows with spots, large patches or areas of fusion (Figure 1a).In CAP group, 47 patients (39.8%) had ground-glass shadows in the lungs, 71 patients (60.2%) showed consolidation, and the density differences between the two groups was signi cant (χ 2 = 48.75,P < 0.001).In COVID-19 group, 54 cases (32.8%) exhibited a "crazy-paving" pattern mainly characterized by interlobular septal thickening (Figure 3B) that differed from the subpleural reticular or honeycomb changes observed in the cases (28.8%) of CAP group.Eight (4.8%) patients in COVID-19 group showed "wandering" lung lesions (Figure 3c-d).
In addition to the newly formed exudative in ammatory lesions mentioned above, the chest CT in CAP group was revealed chronic obstructive manifestations, such as emphysema, bullae, and "mosaic" signs or small airway obstructive changes.CAP group included 68 patients (57.6%) with brous cords or pulmonary texture aggregates, representing a signi cantly higher number of patients than that found in COVID-19 group (5 patients, 3.1%) (χ 2 = 107.14,P < 0.001); bronchial wall thickening was also observed in CAP group (37 cases, 31.4%), and 18 cases (10.1%) were observed in COVID-19 group.The difference between the two groups was signi cant (χ 2 = 18.4,P < 0.001).In CAP group, 12 patients (10.2%) exhibited small cavities, and 8 cases exhibited pulmonary bullae, representing a signi cantly higher number than the corresponding number of patients in COVID-19 group.

Relationship between the clinical characteristics and imaging manifestations
The total number of lung lobes involved in the COVID-19 and CAP groups was 384 and 257, respectively.
The number of single lesions in COVID-19 group was signi cantly higher than that in CAP group (χ 2 = 8.38, P = 0.03), and the number of multi-lobar foci in CAP group was signi cantly higher than that in COVID-19 group (χ 2 = 6.68,P = 0.01).The lesions in both groups were mainly located in the middle and outer lobes.In total, 148 patients (89.7%) in COVID-19 group and 62 patients (52.5%) in CAP group had lesions located in the outer zone.There was a signi cant difference between the two groups (χ 2 = 49.6,P < 0.001).The statistical analysis showed that there was a positive correlation between the age of onset and the number of involved lobar segments (r = 0.62, P < 0.001).
Of the above clinical and imaging manifestations, the age factor, total number of leukocytes, and groundglass shadows have the important clinical signi cance in the diagnosis of COVID-19 (Table 3).The characteristics of CAP group were consolidation in the lung lesions, accumulation of brous cords or textures and thickening of the bronchial wall (Table 4).

CT follow-up of COVID-19
In total, 135 patients in COVID-19 group were re-examined 3-7 days after the rst CT examination.In total, 32 patients (23.70%) showed increases in the lesion size and number and were classi ed as progressing, 25 patients (18.5%) were stable, and 78 patients (57.8%) were improved.
All patients with COVID-19 underwent CT re-examination one month after treatment, and 15 patients (15.6%) showed no abnormalities in either lung at the time of re-examination (Figure 3e-f).The remaining 87 patients (52.7%) showed localized strip shadows, 43 patients (26.1%) showed extensive brosis, 10 patients (6.1%) had limited aky shadows, 6 patients (3.6%) had wide strip shadows, and 4 patients (2.4%) had diffuse grid shadows (Figure 3g-h).Regarding the relationship between age and prognosis, in the patients aged < 60 years with COVID-19, the late CT changes were not signi cantly related to age; in the patients aged over 60 years, the CT sequelae changed with increasing age (Figure 4 and Supplementary Table 2).

Discussion
Currently, retrospective studies investigating various aspects of COVID-19 are being conducted.The infectivity of SARS-CoV-2 is high, the source of the pathogen is unknown, there is no speci c treatment method, and the mortality rate is high [15]; therefore, the early diagnosis of COVID-19 is a challenge.A correct and timely diagnosis is of great signi cance for treatment and prevention of dissemination.Due to delayed laboratory results and the limited sensitivity of the tests, the controversy regarding how to obtain an early clinical diagnosis remains [16].Thoracic imaging plays an important role in early diagnosis.We believe that in epidemic areas, diagnosis with imaging characteristics is the key to distinguish COVID-19 from other CAPs.
The data were similar to most clinical observations [17][18][19].COVID-19 group mostly comprised young adults as follows: 129 patients (78.2%) were aged 20-60 years, while only 18 patients (15.3%) were aged 20-60 years in CAP group.This distribution does not comply with the general spread of infectious diseases, i.e., children and elderly populations are often the susceptible groups.Young adults typically have the strongest resistance to infectious diseases, and the incidence rate should be low.Among the 72 314 cases noted by the Chinese Center for Disease Control and Prevention, the incidence rate in China among those aged under 10 years was less than 1% [20].The reason for the high incidence rate in young adults cannot exclude the possibility of more virus contact and infection opportunities due to more social interactions.Notably, further studies investigating the epidemiology and pathogenesis of this disease are needed.Fever was the prominent clinical manifestation in both groups, but the increase of temperature in COVID-19 group was more obvious than that in CAP group.During the early stage of the disease (within seven days), the total number of leukocytes in the observed patients with COVID-19 was normal, low (< 4 × 10 9 /L), or showed a decreasing trend, which is similar to that reported in the literature [21] and may be caused by a decrease in lymphocytes during the rst few days after infection.The symptoms of muscle soreness and fatigue in COVID-19 group were signi cantly more prevalent than those in CAP group, while the symptoms of expectoration in CAP group were signi cantly more prevalent than those in COVID-19 group, which may be related to the fact that CAP is prone to producing more purulent secretion due to bacterial infection [22].The results showed that the incidences of heart disease, hypertension, chronic lung disease and diabetes in CAP group were signi cantly higher than those in COVID-19 group.
Our study shows that COVID-19 chest CT reveals ground-glass shadows of different sizes, and the most typical was round or quasi circular.This shadow appeared in 32.1% (53 cases) of the patients, which is similar to other studies [23,24].In addition, the incidence of a single lesion in COVID-19 group was signi cantly higher than that in CAP group, which may be related to the early stage of SARS-CoV-2 infection.With the progression of the disease, the GGO in some patients expanded and gradually developed into ground-glass shadows with multiple patches, large areas of fusion or large and small patches coexisting, but the density change was relatively small; this nding is in contrast to the rapid consolidation observed as CAP progresses.This important feature of COVID-19 has not been reported.Notably, in some patients with mild CAP, the lung lesions did not expand until absorption.Another 32.7% (54 cases) of patients with COVID-19 showed ne reticular shadows overlapping with the ground-glass shadow, which may be related to alveolar oedema in the lung lesion area and slight thickening of the alveolar septum with in ltration of monocytes, lymph and plasma cells [25].
In CAP group, new exudative in ammatory lesions were often accompanied by obvious brous components or texture aggregation and cord adhesion in other parts of the lung, which manifested as bronchitis or bronchial thickening and blurring of the outer edge, suggesting that these patients had previously experienced the process of lung in ammation and that there were some remaining chronic in ammation or later changes.This nding is quite different from the acute onset, rapid progress and multiple manifestations of lung in ammation observed with COVID-19.Therefore, this study suggests that the lung ndings of brous cord and bronchial wall thickening can be used to exclude COVID-19.In addition, a certain proportion of emphysema, pulmonary bullae, reticular or honeycomb changes under the pleura, "mosaic" signs and/or small cavity shadows in the consolidation area were observed in CAP group.In conclusion, the diversity of lung lesions and the coexistence of new and old lesions in CAP group are helpful in distinguishing CAP from COVID-19.
Six patients with COVID-19 underwent chest CT re-examinations approximately one week after the onset of the disease.The lesions showed "wandering" characteristics, which may indicate heterogeneity in the pathological changes in different lung areas; thus, early changes and changes in the organizing phase of diffuse alveolar injury can appear in different segments simultaneously, which occurs more commonly in young patients.This nding is a new nding for the diagnosis of COVID-19.However, due to the small number of patients in this group, whether these ndings have a good diagnostic speci city remains to be further con rmed.
This study found that a positive correlation exists among age, the size of the lesion and total number of lung segments involved in COVID-19 group.The correlation coe cient between age and the size of the lesion was 0.522 (P < 0.001), and the correlation coe cient between age and the total number of lung segments involved was 0.531 (P < 0.001).In COVID-19 group, the elderly patients had a signi cantly wider range of lesions in the rst visit than the young patients, and the number of involved segments in COVID-19 group was signi cantly higher than that in CAP group, which may be due to related reasons, such as a weak constitution and many basic diseases among elderly patients.The subsequent follow-up of COVID-19 group showed that the patients aged over 60 years had many changes remaining in the lung, and the most serious manifestation was extensive brous cord shadows in the lung.In this group, 32 patients (19.4%) were aged over 60 years, and the prognosis of these patients was poor.The 2-to 3month follow-up showed that most remaining changes in the lung could be absorbed, but the absorption was slow.
This study has several limitations.First, we retrospectively analysed the imaging data of the two groups of patients.Our screening strategy has selection bias.Currently, a more balanced and large-scale prospective study involving similar patients is still needed.In this study, the different situations of the disease in each centre and the different experiences of the radiologists had some in uence on the diagnosis of the disease.In addition, some patients had positive results on the new crown pneumonia nucleic acid test.However, during the winter and spring seasons, these patients often have viral and bacterial pneumonia or other diseases, which may cause some interference with the performance of CT in evaluating new crown pneumonia.

Conclusion
In conclusion, the most common CT manifestations of COVID-19 are simple GGO, GGO with consolidation or grid-like changes.In clinical work, a CT examination should be used in combination with the epidemiological history, clinical characteristics and haematological examination to improve the accuracy of a covid- 19     another ground-glass-like density shadow appeared in the middle lobe of the right lung, and the lesions in the lungs showed "wandering" characteristics; e-f.A 32-year-old female patient was re-examined after 10 days of treatment.CT showing that the multiple lung lesions disappeared; g-h.A 68-year-old female patient was re-examined after 15 days of treatment.CT showing that the diffuse lesions of both lungs were absorbed into extensive brosis.

2. 1
Patient cohort From January 21 to February 28, 2020, the clinical data of 165 patients with COVID-19 from seven hospitals in Anhui Province, China, including 76 males and 89 females aged 5 to 91 years with an average age of 45.1 ± 17.6 years, were reviewed.The diagnosis complied with the Guidelines for the Diagnosis and Treatment of New Coronavirus Pneumonia (Seventh Edition) formulated by the National Health Committee of the People's Republic of China [14].In total, 118 patients with CAP were selected from the First A liated Hospital of the University of Science and Technology of China for pneumonia during the same period.The pathogens were con rmed in all patients by the RT-PCR detection of sputum throat swabs and blood or other pathogenic tests.The patients with CAP included 48 males and 70 females aged 1 to 76 years with an average age of 15.6 ± 21.4 years.The CT images and clinical data of all patients were collected.The inclusion and exclusion processes are shown in Figure 1, and the results of the community-acquired pneumonia respiratory pathogen panel (CAP-RPP) are shown in Figure 2.

Figure 2 Distribution
Figure 2

Figure 3 CT
Figure 3

Table 2 .
diagnosis and distinguish COVID-19 from CAP.Comparison of various chest CT manifestations in COVID-19 and CAP patients

Table 3 .
Statistical prediction of some clinical and CT features for the diagnosis of COVID-19, % (n/n)