A novel strategy for screening mutations in the voltage-gated sodium channel gene of Aedes albopictus based on multiplex PCR-mass spectrometry minisequencing technology

Table 3 Primer sequences for PCR amplification of Aedes albopictus VGSC gene target sites

Target site	Primer sequences^a
Target site	Forward primer sequence	Reverse primer sequence
1016	1016-F: acgttggatgCCACCGTAGTGATAGGAAATCT	1016-R: acgttggatgGTTGGCGATGTTGGACTTGA
1532/1534	3234-F acgttggatgGCGTACCTGTGTCTGTTC	3234-R: acgttggatgTTCTTCTTCTGCTCGTTGA

^aThe 10-bp fixed sequence added to the 5' end of the primer is indicated by lowercase letters

ISSN: 2049-9957