Table 4 Primer sequences for Aedes albopictus VGSC gene MPE for target site detection
Target site | Mass probe name | Mass probe sequence (5'-3') | Mass probe mass (Da) | Extension base of nonvariant | Extended mass of MPE of nonvariant (Da) | Extension base of variant | Extended mass of MPE of variant (Da) | Extension base of variant | Extended mass of MPE of variant (Da) | Extension base of variant | Extended mass of MPE of variant (Da) |
|---|---|---|---|---|---|---|---|---|---|---|---|
1016 | vg1016 | GGCTAAGAAAAGGTTAAGT | 5924.8 | T | 6221.8 | G | 6197.8 | – | – | – | – |
1532 | it1532 | CTACTTCGTGTTCTTCA | 5102.4 | T | 5444.4 | C | 5375.4 | – | – | – | – |
1534 | fl15341 | GTTGAGGGTGAAGAACGACCCGA | 7162.6 | T | 7459.6 | C | 7475.6 | – | – | – | – |
fl15341-2 | TACTTCGTGTTCTTCATCATC | 6313.2 | T | 6655.2 | C | 6586.2 | – | – | – | – | |
sc1534 | TTGAGGGTGAAGAACGACCCG | 6520.2 | T | 6817.2 | C | 6833.2 | G | 6793.2 | – | – | |
sc1534-2 | TCTACTTCGTGTTCTTCATCATCT | 7210.8 | T | 7552.8 | C | 7483.8 | G | 7523.8 | – | – | |
fl15342 | AGGGTGAAGAACGACCC | 5253.4 | C | 5566.4 | G | 5526.4 | A | 5595.4 | T | 5550.4 |