The in vitro siRNA treatment of adult female B. malayi leads to a reduction in microfilaria release. Tubes containing six adult worms each were treated with 5 μM siRNA for different time periods (12, 24, 36, 48, 60 hours). After 60 hours of treatment, six females were cultured for a further two days in fresh siRNA-free normal culture medium (108 hours). The percent reduction in the mf release was analyzed in the Bm-iPGM-specific siRNA-treated and negative control group over that of the control media group. There was a significant reduction in the release of mf by Bm-iPGM siRNA-treated worms starting from 12 hours and continuing until 108 hours. The criterion for statistical significance between the results was carried out by ANNOVA and was expressed in the form of p value that was considered significant and marked as p <0.05 *, p <0.01 as highly significant **, and p <0.001 *** as very highly significant. Data represent mean ± SE of percent reduction in mf counts from two independent experiments.