Fig. 1From: High-throughput screening against thioredoxin glutathione reductase identifies novel inhibitors with potential therapeutic value for schistosomiasisValidation of the HTS assay. a Concentration-dependent inhibitory activity of naphthazarin on SmTGR under the optimized assay conditions, from which the IC50 value was calculated (n = 3, mean ± SEM). b Z' factor determination. Assays were performed under the optimized conditions and 160 replicates of total and background signals were studied. HTS assay parameters, including Z' factor and S/B were examined. Raw fluorescence units (Y-axis in the left) from each of the controls and % inhibition (Y-axis in the right) are shown. Dashed lines indicate means and mean ± 3 × standard deviation of the 160 data pointsBack to article page