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Table 1 PCR primers for blood source identification

From: Ecological niches and blood sources of sand fly in an endemic focus of visceral leishmaniasis in Jiuzhaigou, Sichuan, China

Species Primers Sequence (5'to3') Amplicon length (bp)
Universal reverse UR GGT TGT CCT CCA ATT CAT GTT A  
Human (Homo sapien) HF + UR GGC TTA CTT CTC TTC ATT CTC TCC T 334
Pig (Sus domesticus) PF + UR CCT CGC AGC CGT ACA TCT C 453
Cow (Bos taurus) CF + UR CAT CGG CAC AAA TTT AGT CG 561
Dog (Canis lupus) DF + UR GGA ATT GTA CTA TTA TTC GCA ACC AT 680
Chicken (Gallus gallus) ChF CAT ACT CCC TCA CTC CCC CA 802
ChR CCC CTC AGG CTC ACT CTA CT
Rabbit (Oryctolagus cuniculus) RF CGA TAC CTC CAC GCT AAC GG 388
RR TTG GGT TGT TGG AGC CAG TT
  1. Note: F Forward, R Reverse. Universal reverse primer was used in couple with respective forward primers