Fig. 1

Methodological validation of the HPLC-HRMS approach using standard preparations of R-PZQ, S-PZQ and mebendazole in blank plasma. Smoothed curves (SM) resulting after a total running time (RT) of 25.00 min; RT = retention time at peak of the signal; AA = area under the curve; mebendazole was used as internal stanard (IS). The mass-to-charge ratio (m/z) made it possible to calculate the mass of the compounds and thus identify the findings in each run: R-PZQ (313.1880–313.1942), S-PZQ (313.1880–313.1942) and mebendazole (296.1000–296.1060). R-PZQ was faster than S-PZQ so ion peaks of R-PZQ were in the first line