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Table 4 Pooling experiment with urine samples from River Basin children

From: Utilizing the ultrasensitive Schistosoma up-converting phosphor lateral flow circulating anodic antigen (UCP-LF CAA) assay for sample pooling-strategies

Study site River Basin, Senegal
Included 81 children (7–12 years of age) with high susceptibility of being infected
Prevalence before PZQ 89% (72 children) based on individual UCAA10 diagnosis
  100% (81 children) based on individual UCAA250 diagnosis
CAA level 1 519 pg/ml
Prevalence after PZQ 43.2% (35 children) based on individual UCAA10 diagnosis
  86.4% (65 children) based on individual UCAA250 diagnosis
CAA level 1 33 pg/ml
POOLING Pooling of samples collected after PZQ treatment
Pool composition Nine pools, each comprising an equal volume of 9 children –‘9 sample pool’
One pool comprising an equal volume of all 81 samples – ‘81 sample pool’
Applied test Pools were analysed with the UCAA250 format
CAA level 9-sample pool 1 47, 84, 29, 57, 60, 36, 22, 40, 33 pg/ml – average of nine ‘9 sample pools’ 45 pg/ml
CAA level 81-sample pool 1 40 pg/ml
  1. 1All concentrations indicate the average amount of CAA per individual, either of the whole selection (n = 81) or for the number of individuals in a specific pool