Table 1 Primers designed for multiplex PCRs and sequencing
Drug | Gene | Primer | Sequence (5′ → 3′) | Amplicon size (bp) |
|---|---|---|---|---|
RIF | rpoB | rpoB-F | GGTCGCCGCGATCAAGGAGT | 228 |
| Â | Â | rpoB-R | GAGCCGATCAGACCGATGTT | Â |
INH | katG | katG-F | CAGATGGGCTTGGGCTGGAA | 152 |
| Â | Â | katG-R | TTCGTCAGCTCCCACTCGTAGC | Â |
| Â | inhA | inhA-F | TGGTCGAAGTGTGCTGAGTC | 193 |
| Â | Â | inhA-R | TCCGGTAACCAGGACTGAAC | Â |
| Â | oxyR-ahpC | oxyR-ahpC-F | GCAGTCACAACAAAGTCAGCTCTG | 401 |
| Â | Â | oxyR-ahpC-R | ACAGGTCACCGCCGATGAGA | Â |
SM | rpsL | rpsL-F | TTGTGGTTGCTCGTGCCTG | 635 |
| Â | Â | rpsL-F | CAACTGCGATCCGTAGACCG | Â |
| Â | rrs | rrs-R | CTCTCGGATTGACGGTAGGTGG | 540 |
| Â | Â | rrs-F | GCGTCCTGTGCATGTCAAACC | Â |
EMB | embB | embB-F | CGTGGTGATATTCGGCTTCCTG | 493 |
| Â | Â | embB-R | CTGCACACCCAGTGTGAATGCG | Â |