Table 2 Primers for PCR amplification for mitochondrial genes of Echinococcus multilocularis with positions based on a reference sequence (AB018440 in GenBank)
Gene | Primer name | Primer sequence (5′–3′) | Length | Position | Size (bp) | References |
|---|---|---|---|---|---|---|
nad5 | Emnd5-F | CTATTATGGTGTTAGTTGTTGAC | 23 | 490–512 | 1914 | [57] |
Emnd5-R | AACCACAGACATATCTATATCG | 22 | 2382–2403 | |||
nad1 | Emnd1-F | GAGTTTGCGTCTCGATGATAGG | 22 | 7386–7407 | 1126 | [57] |
Emnd1-R | TCCCCAAAACCCACATTCTAC | 21 | 8491–8511 | |||
cox1 | Emco1-F | AGGTTTGACTTTCTCTTTGGTT | 22 | 9072–9093 | 1801 | [57] |
Emco1-R | GGCAAATAAACCTAAACAACC | 21 | 10,852–10,872 | |||
cob | Emcob-F | GTTTAAACTGGTAGATTGTGGTTC | 24 | 3055–3078 | 1323 | [25] |
Emcob-R | CTCCACAGTAGAAATCACCATCA | 23 | 4377–4355 | |||
cox3 | Emco3-F | TAAATATAGAACGAAAGTAAAT | 22 | 2180–2201 | 1012 | This study |
Emco3-R | TACAAACCCACTACTTCAATAA | 22 | 3191–3170 | |||
nad4L | Emnd4L-F | ATTGACTTATTTAGGTGGGTGT | 22 | 4073–4094 | 1364 | This study |
Emnd4L-R | TAGTCGGAAATGAACATAACCT | 22 | 5436–5415 | |||
nad3 | Emnd3-F | GATTATGGGGAGTCTGAAAGGG | 22 | 8088–8109 | 1179 | This study |
Emnd3-R | TAAACCAACAAAACCAGACCAT | 22 | 9266–9245 | |||
nad6 | Emnd6-F | GGTGGGGAAAATCAGGCGGTTG | 22 | 12,728–12,749 | 959 | This study |
Emnd6-R | AACACCAAAAAACCAAACACTA | 22 | 13,686–13,665 | |||
nad4 | ND4Em-F | TACTGTGGAGATTATTATTAGG | 22 | 4368–4389 | 1519 | This study |
ND4Em-R | CAAAATCATTCACAATAACCAT | 22 | 5886–5865 | |||
atp6 | ATPEm-F | AGTGATGGTTTAATGAGGTGTT | 22 | 5668–5689 | 799 | This study |
ATPEm-R | ACAACACAAAAAACAAAACAAC | 22 | 6466–6445 | |||
nad2 | ND2Em-F | TTTTCGATTGATCATTAGGTTG | 22 | 6363–6384 | 1179 | This study |
ND2Em-R | CAAACTAAGCAATAAGCCAAAA | 22 | 7541–7520 | |||
cox2 | Co2Em-F | CAGTAGACTTTTTTGTTGAATG | 22 | 11,798–11,819 | 1489 | This study |
Co2Em-R | AAACCTCCAACAACATAAATCT | 22 | 13,286–13,265 |