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  • Research Article
  • Open Access

Estimating the prevalence of schistosomiasis japonica in China: a serological approach

Infectious Diseases of Poverty20187:62

https://doi.org/10.1186/s40249-018-0443-2

  • Received: 10 November 2017
  • Accepted: 17 May 2018
  • Published:

Abstract

Background

The prevalence of schistosomiasis japonica has decreased significantly, and the responses changing from control to elimination in Jiangsu Province, P.R. China. How to estimate the change in prevalence of schistosomiasis using only serological data will be important and useful.

Methods

We collected serum samples from 2011 to 2015 to build a serum bank from Dantu County of Jiangsu, China. Serum samples were detected by enzyme-linked immunosorbent assay (ELISA), the positive rate and optical density (OD) value were obtained. The Bayesian model including the prior information of sensitivity and specificity of ELISA was established, and the estimated infection rates were obtained for different years, genders and age groups.

Results

There was no significant difference in the mean OD between different years and genders, but there was a significant difference between the different age groups. There were statistically significant differences in the positive rate for different years and age groups, but no significant difference at different genders. The estimated infection rate for the five years was 1.288, 1.456, 1.032, 1.485 and 1.358%, respectively. There was no significant difference between different years and between genders, but a significant difference between different age groups.

Conclusions

The risk of schistosomiasis transmission in this area still exists, and risk monitoring of schistosomiasis should be strengthened.

Keywords

  • Enzyme-linked immunosorbent assay (ELISA)
  • Bayesian statistics
  • Schistosomiasis japonica
  • Sensitivity
  • Specificity
  • Estimated infection rate

Multilingual abstract

Please see Additional file 1 for translation into in the five official working languages of the United Nations.

Background

In tropical and subtropical regions, schistosomiasis remains as an important public health problem. Up to now, roughly 800 million people are at risk of schistosomiasis infection and more than 200 million people become infected [1, 2]. Over the past 50 years, the prevalence of schistosomiasis had decreased significantly, and the number of infections has greatly reduced in China [36]. Jiangsu Province is located in the lower reaches of the Yangtze River and has historically been one of the most affected regions of schistosomiasis in China. The cumulative number of patients was 253.07 million, and the cumulative area of the snail habitat was 1.47 billion square meters [7]. After 60 years of active and effective prevention and control, the prevalence of schistosomiasis finally fell below the government standard of 1% in 2010 [8, 9].

Dantu County located in the southern aspect of Jiangsu Province and along the Yangtze River (Fig. 1), has been one of the most endemic counties (districts) with schistosomiasis in Jiangsu Province [10]. At the end of 2005, the cumulative number of all schistosomiasis patients was 4.85 million, and the area of living and the infected snail was 2.8634 and 0.551 km2, respectively. Acute schistosomiasis cases were also found every year from 2002 to 2005 [11]. The area of snail was 2.756 million square meters in 2011, and 766 000 square meters in 2015. The areas declined significantly, especially in the marshland and mountainous regions. The expanded chemotherapy was implemented on potential cases of schistosomiasis, and the number decreased from 159 to 45 in 2015 between 2011 and 2015.
Fig. 1
Fig. 1

The location of Dantu County, Jiangsu Province, People’s Republic of China

In the early stages of schistosomiasis control, stool examination was improved by the implementation of the Kato-Katz thick smear method for schistosomiasis surveillance [12]. With the successful implementation of prevention and control project, the prevalence of schistosomiasis markedly decreased. But given the low sensitivity of the stool examination tests, the recent low levels of infection in the population have made an epidemiological investigation and estimation of the infection rate was very difficult [13]. With the rapid development of immunology and molecular biology techniques, some immunodiagnostic kits such as enzyme-linked immunosorbent assay (ELISA) or dipstick dye method have been developed and used in the field [13, 14]. In our previous study, ELISA test was proved with higher efficacy,and ELISA was selected for blood eximnation [15].

In this study, blood samples were collected from a serum bank for five consecutive years (2011–2015) and examined by ELISA. A serological approach was built to evaluate the effect of prevention and treatment.

Methods

Study region and samples selection

In this study, Wudun Village of Dantu County in Jiangsu Province is selected. The village is located on the middle island of Yangtze River. The village’s population is 2284 and has 809 households. The village is in an endemic area of schistosomiasis. By the end of 2017, the cumulative area of the snail habitat were 23.031 million square meters and the cumulative people of schistosomiasis were 2136 [16]. Blood samples from residents older than 6 years old were collected from October to November each year from 2011 to 2015. Other demographic information including name, gender and age was also collected at the same time. [17] All samples were stored in a − 70 °C frozen storage refrigerator in Jiangsu Institute of Preservation Diseases (JIPD). The standard process was used to prevent repeated freezing and thawing and ensure the quality of serum samples.

Samples testing

In this study, the schistosomiasis antibody kit of ELISA that was used produced by Shenzhen Huakang Bio-Biomedical Engineering Co., Ltd. (Product batch number: 20160101). The value of optical density (OD) for ELISA was measured by a microplate reader. The OD value of all specimens was subtracted from the OD value of the blank control to obtain the true value of samples. A unified batch number of reagents was used to detect serum samples. Before the laboratory testing work, the person who participated in the investigation were trained. The training content included the use of ELISA reagents, read-out of results and OD value readings.

Establishment of Bayesian model

The sensitivity and specificity of ELISA for the different age and different gender groups were calculated from a previously filed study [18]. Blood and stool samples were collected from 6 to 65-year-old residents. The Kato-Katz method was used to test stool samples, in which one stool sample was checked three times. ELISA method was used to test blood samples. We then used the Wilson interval algorithm to obtain a 95% confidence interval (CI) for sensitivity and specificity [19, 20].

In the process of Bayesian model construction, the prior distribution of sensitivity and specificity is assumed to be a beta (α, β) distribution [21, 22]. The beta distribution is a probability density distribution function between 0 and 1. Where π is the mean of the prior distribution of sensitivity or specificity, replaced by its prior central value. δ is the a priori standard deviation and is replaced by a quarter of its a priori range. The formula of α and β is:
$$ a=\pi \left[\frac{\left(1-\pi \right)\pi }{\delta^2}-1\right] $$
$$ \upbeta =\left(1\hbox{-} \uppi \right)\left[\frac{\left(1\hbox{-} \uppi \right)\uppi}{\updelta^2}\hbox{-} 1\right] $$

In addition, assuming a priori information without infection rate, a priori distribution is beta (1, 1). The variance \( {\delta}_k^2 \) , \( {\updelta}_{\mathrm{j}}^2 \) and \( {\updelta}_{\mathrm{i}}^2 \) of the normal distribution of age, gender and village random effects are subject to the no-information back-gamma distribution.

According to the prior distribution of sensitivity and specificity, Bayesian models were established only using the serological data to estimate the infection rate at different age and gender groups. The Bayesian model analyses were conducted in WinBUGS (Imperial College and MRC, London, UK), (http://www.mrc-bsu.cam.ac.uk/software/bugs/the-bugs-project-winbugs/) is neither nor allowed to have any missing value, so was subject to the following binomial distribution:
$$ {\mathrm{t}}_{\mathrm{k}}\sim \mathrm{Binomial}\left({\mathrm{p}}_{\mathrm{k},}{\mathrm{n}}_{\mathrm{k}}\right) $$
$$ {\mathrm{p}}_{\mathrm{k}}={\uppi}_{\mathrm{k}}{\mathrm{s}}_{\mathrm{j}}+\left(1\hbox{-} {\uppi}_{\mathrm{k}}\right)\left(1\hbox{-} {\mathrm{c}}_{\mathrm{j}}\right) $$

Where nkand tk represent the population and positive rate of ELISA. The pk and πk represent infection rate of population and positive in the k age group, and the meanings of sj and cj were consistent with previous content.

The polynomial distribution was adjusted to:
$$ {\mathrm{p}}_{\mathrm{k}}={\uppi}_{\mathrm{k}}{\mathrm{s}}_{\mathrm{j}}\mathrm{z}\left[\mathrm{i}\right]+\left(1\hbox{-} {\uppi}_{\mathrm{k}}\mathrm{z}\left[\mathrm{i}\right]\right)\left(1\hbox{-} {\mathrm{c}}_{\mathrm{j}}\right) $$
$$ \mathrm{z}\left[\mathrm{i}\right]=\mathrm{dbern}\left({\updelta}_{\mathrm{z}}\right) $$
$$ {\updelta}_{\mathrm{z}}\sim \mathrm{beta}\left({\upalpha}_{\mathrm{z}},{\upbeta}_{\mathrm{z}}\right) $$

Where z [i] is the adjustment parameter for different villages. αz and βz is the prior distribution of the z [i].

Concerning the data structure, the information came from different layers, the first, second and third layer was studied year, gender and age group, respectively. The model was established as the following, and included the random effect at different layer [23]:
$$ \mathrm{logit}\left({\uppi}_{\mathrm{k}}\right)=\kern0.5em {\mathrm{uj}}_{\mathrm{k}} $$
$$ {\mathrm{uj}}_{\mathrm{k}}\sim \mathrm{normal}\left({\mathrm{ui}}_{\mathrm{j}},{\updelta}_{\mathrm{k}}^2\right) $$
$$ {\mathrm{u}\mathrm{i}}_{\mathrm{j}}\sim \mathrm{normal}\left({\mathrm{u}}_{\mathrm{i}},{\updelta}_{\mathrm{j}}^2\right) $$
$$ {\mathrm{u}}_{\mathrm{j}}\sim \mathrm{normal}\left(0,\kern0.5em {\updelta}_{\mathrm{i}}^2\right) $$

The ujk, uij and ui represent the random effects of age, gender and year which were following the normal distribution, and were used to quantify the infection rate. The \( {\updelta}_{\mathrm{k}}^2 \), \( {\updelta}_{\mathrm{j}}^2 \) and \( {\updelta}_{\mathrm{i}}^2 \) are the variance of the corresponding variables.

According to the random effects of each gender uij and each year ui, the infection rate of each gender πj and the infection rate of each year πi can be calculated:
$$ {\uppi}_{\mathrm{j}}=\frac{\exp \left({\mathrm{u}\mathrm{i}}_{\mathrm{j}}\right)}{1+\exp \left({\mathrm{u}}_{\mathrm{i}}\right)} $$
$$ {\uppi}_{\mathrm{i}}=\frac{\exp \left({\mathrm{u}}_{\mathrm{i}}\right)}{1+\exp \left({\mathrm{u}}_{\mathrm{i}}\right)} $$

Ethics statement

All studies described here were approved by the Ethics Review Committee of Jiangsu Institute of Parasitic Diseases, China (Permission number: JIPDERC2010008). The field studies did not involve endangered or protected species.

Results

Sensitivity and specificity of different gender and age groups

Table 1 shows the sensitivity and specificity of the serological test for different age and gender. On the whole, the sensitivity of both men and women increased with age. There was no significant difference in sensitivity (F = 2.426, P > 0.05) and specificity (F = 1.577, P > 0.05) between different genders. There was significant difference in sensitivity (F = 16.231, P < 0.01) and specificity (F = 7.727, P < 0.01) between different age groups. Among those 6 to 30 years old, the sensitivity of males and females improved as age increased. Its specificity showed a downward trend, and the specificity among males was lower than among females of the same age (Figs. 2 and 3).
Table 1

Prior distribution of Sensitivity and Specificity in ELISA of males and females in Dantu County, Jiangsu Province, People’s Republic of China

Age group

Sensitivity

Specificity

Sensitivity

Specificity

Mean (2.5% CI, 97.5% CI)

Mean (2.5% CI, 97.5% CI)

α

β

α

β

Males

      

6~

72.81% (59.81,85.81%)

88.83% (87.60, 90.06%)

33.38841

12.468

2329.468

292.921

10~

94.15% (91.04, 97.26%)

69.51% (68.26, 70.75%)

213.5133

13.267

3801.042

1667.69

20~

91.95% (83.89, 100.00%)

51.05% (48.46, 53.63%)

41.06135

3.597

763.048

731.806

30~

99.30% (98.60, 100.00%)

41.19% (39.61, 42.76%)

562.4636

3.965

1608.251

2296.693

40~

96.37% (94.91, 97.83%)

35.51% (34.28, 36.73%)

631.6588

23.793

2166.815

3936.029

50~

94.71% (92.59, 96.82%)

30.61% (29.49, 31.73%)

423.7224

23.691

2072.924

4699.124

60~

98.96% (97.91, 100.00%)

31.83% (30.23, 33.42%)

373.8274

3.948

1085.359

2325.038

Females

      

6~

50% (29.93, 70.07%)

89.51% (88.07, 90.95%)

11.91296

11.913

1620.368

189.897

10~

87.3% (80.74, 93.85%)

81.91% (80.82, 82.99%)

89.25677

12.991

4123.763

911.049

20~

94.33% (88.65, 100.00%)

69.91% (67.86, 71.95%)

61.76833

3.716

1405.945

605.277

30~

89.75% (85.75, 93.75%)

49.31% (47.92, 50.69%)

205.5135

23.471

2569.355

2641.79

40~

99.37% (98.74, 100.00%)

46.31% (45.21, 47.41%)

625.9521

3.969

3805.965

4412.486

50~

95.49% (93.07, 97.90%)

50.86% (49.69, 52.03%)

281.373

13.305

3713.784

3588.19

60~

94.33% (88.65, 100.00%)

55.04% (53.28, 56.80%)

61.76833

3.716

1758.255

1436.249

Fig. 2
Fig. 2

Sensitivity of males and females in different age groups in Dantu County, Jiangsu Province, People’s Republic of China

Fig. 3
Fig. 3

The specificity of males and females in different age groups in Dantu County, Jiangsu Province, People’s Republic of China

The prior distribution of sensitivity and specificity

The sensitivity and specificity among males were between (0.7500, 1.0000) and (0.3059, 0.8898), and the sensitivity and specificity among females were between (0.000, 1.0000) and (0.4631, 0.8960). The 95% CI for sensitivity and specificity was obtained using the Wilson interval algorithm, and a prior distribution of sensitivity and specificity was obtained for different age groups and gender (Table 1).

Results of serological testing

In this study, 2180 blood samples were collected from 2011 to 2015, including 1132 samples from males and 1048 samples from females. The highest positive infection rate was 37.38% in 2012, and the lowest rate was 7.36% in 2015 (Table 2). The positive rate decreased year by year since 2012 (Fig. 4). The number of samples from males in 2011 was less than females in 2015, but the number of samples from males were greater than females in 2012, 2013 and 2014. The male positive rate from serology testing was lower than that of females in 2011, 2012 and 2014, but the male positive rate of serology test was higher than female in 2013 and 2015 (Fig. 4). The positive rate increased in the overall population as age increased from 40 years of age. The highest positive rate was among those 60 years old and older. There were statistically significant differences in the positive rate of serological tests at the monitoring point for all five consecutive years (P = 0.0001) and age groups (P = 0.0001). There was no significant difference in the positive rate of serology between different gender (P = 0.79).
Table 2

The positive rate of ELISA from2011 to 2015 in Dantan County, Jiangsu province, People’s Republic of China

Year

Gender

The positive

The negative

Positive rate(%)

Total

2011

Male

26

141

15.569

167

Female

34

160

17.526

194

Total

60

301

16.621

361

2012

Male

75

134

35.885

209

Female

79

124

38.916

203

Total

154

258

37.379

412

2013

Male

42

164

20.388

206

Female

32

154

17.204

186

Total

74

318

18.878

392

2014

Male

66

239

21.639

305

Female

54

153

26.087

207

Total

120

392

23.438

512

2015

Male

25

220

10.204

245

Female

12

246

4.651

258

Total

37

466

7.356

503

Fig. 4
Fig. 4

The positive rate of ELISA from 2011 to 2015 in Dantu County, Jiangsu Province, People’s Republic of China

The average OD value for 2011–2015 was 0.087, 0.287, 0.078, 0.260 and 0.065 from 2011 to 2015, with the highest in 2012 and the lowest in 2015. The OD values were similar between male and female groups (Fig. 5) with no significant difference (P = 0.113). The OD value gradually increased with age, and the average OD was the largest among those more than 60 years of age (Fig. 6). This difference was statistically significant (P = 0.0001), showing the OD value was positively correlated with age, with a correlation coefficient of 0.995 (P < 0.001). There was no significant difference in mean OD between different study years (P = 0.488).
Fig. 5
Fig. 5

The OD value of ELISA from 2011 to 2015 in Dantu County, Jiangsu Province, People’s Republic of China

Fig. 6
Fig. 6

The average OD of the age groups from 2011 to 2015 in of Dantu County, Jiangsu Province, People’s Republic of China

Estimating the infection rate of the population in monitoring points

Table 3 shows the estimating infection rate for different genders and age groups. The infection rate from 2011 to 2015 was 1.288, 1.456, 1.032, 1.485 and 1.358%, with the highest in 2014 and lowest in 2013 (Fig. 7). However, there was no significant difference between different study years (P = 0.998). There was also no significant difference between different gender groups (P = 0.969), but there was a significant difference between different age groups (P < 0.05).
Table 3

The estimate infection rate of the population based on schistosomiasis Bayesian model of Dantu County, Jiangsu Province, People’s Republic of China

Year

Gender

Age group

Infection rate of age groups (%)

Infection rate of gender group (%)

Infection rate of years (%)

2011

Male

10~

1.924

1.043

1.288

30~

1.92

40~

1.96

50~

1.922

60~

1.945

Female

10~

2.021

1.092

20~

2.004

40~

2.085

50~

2.07

60~

2.046

2012

Male

20~

2.413

1.273

1.456

30~

2.429

 

40~

2.38

 

50~

2.456

 

60~

2.41

 

Female

10~

2.701

1.422

30~

2.741

 

40~

2.705

 

50~

2.7

 

60~

2.662

 

2013

Male

20~

1.391

0.778

1.032

30~

1.422

40~

1.368

50~

1.367

60~

1.423

Female

20~

0.766

0.418

40~

0.796

50~

0.783

60~

0.776

2014

Male

10~

2.959

1.577

1.485

30~

2.945

40~

2.939

50~

2.912

60~

2.993

Female

10~

2.674

1.438

40~

2.704

50~

2.702

60~

2.713

2015

Male

40~

2.207

1.154

1.358

50~

2.272

60~

2.166

Female

10~

2.679

1.433

40~

2.795

50~

2.769

60~

2.687

Fig. 7
Fig. 7

Estimation of infection rate of schistosomiasis from 2011 to 2015 in Dantu County, Jiangsu Province, People’s Republic of China

Discussion

According to this national report of schistosomiasis control, the schistosomiasis control processes are changing from working to keep schistosomiasis under control to interrupting transmission, with the final aim of elimination in China [24]. Previous studies indicated that there was a serious misdiagnosis of the pathogenicity method at low levels of prevalence [2527]. In recent years, the Bayesian estimation model has been used to estimate the true infection rate without a gold standard [2830]. So, we explored the use of a serological approach based on consecutive five years of serum library samples to estimate the true prevalence and evaluate the control effectiveness of schistosomiasis japonica. The five-year serum pool was used and no stool samples were collected. It was feasible to use the serum results to estimate the infection rate based on the published literature. Therefore, the infection rate was estimated using five consecutive sera samples. In the process of model building, the sensitivity and specificity of different age groups and gender were included, and then the corresponding parameter values were obtained from field investigation except for Delphi method [31]. This method differed from other detection methods in that there was no leak detection of stool test, and there was not too much false-positive serum to estimate the population infection rate. This approach can significantly improve the accuracy of estimations of disease prevalence and can reduce selection bias and information bias.

The data structure was nested with differing layers: the first layer was studied year, the second layer was the gender group from a different study year, and the third layer was the age group from a different gender group. In order to reflect this data structure, Bayesian hierarchical modelling was used to estimate the infection rates for the different layers, namely the study years, gender and age groups. There is some limitation in using the techniques. First, the sample size which the study calculates specificity and sensitivity may be low, especially some age group have this condition. Secondly, in general, Bayesian estimation model, higher specificity and sensitivity are needed to improve the accuracy of estimation. However, the specificity and sensitivity of some groups in this study are low, which have some impact on the results.

The change in the trend of estimated infection rate appeared similar to the serological detection rate, with increases in trend from 2011 to 2012 and 2013 to 2014, and decreases in trend from 2012 to 2013 and 2014 to 2015. However, there was a significant difference for infection rate from serologic testing (P = 0.0001), but no significant difference for the estimated infection rate (P = 0.998). This suggests that the population of Dantu County in the past five years has had no significant changes. Previous studies have shown that serological tests are difficult to distinguish between the current disease and the previous infection. When the human body is infected with schistosomiasis, the antibody level increases rapidly, then antibodies decrease significantly 2 months after chemotherapy. Antibody levels were not significantly reduced 2 to 8 months after chemotherapy [32].

In this study, the reagents for ELISA came from the same batch, and the reaction conditions were consistent so the OD value can be directly compared. There was no significant difference in mean OD value between different study years, suggesting that the antibody titer did not change throughout the years. The Bayesian model evaluates rate as a whole and does not take into account the individual condition. It is consistent with the OD value. The OD value can be used to assess the change of the epidemic, which is more reliable than the serum positive rate. The lack of significant infection rate differences between genders may be due to the fact that infection opportunities are the same across gender, and there is no difference in the overall level of antibodies between genders. There were significantly different between different age group at the estimated infection rates and the OD value serum samples. This may be due to differential exposure opportunities, given young people rarely have contact with water and other risk factors [33].

Previous studies have shown that this method can be used to monitor the work, and calculate the population infection rate. The main purpose of this study is to evaluate the epidemic situation of schistosomiasis by using the results of previous studies [34, 35]. We found that the risk of schistosomiasis transmission in this area still existed in 2011–2015. The risk monitoring of schistosomiasis needs to be strengthened, and the prevention and control work needs to be further enhanced, certain aspects of disease control can be improved, such as expanding to a more comprehensive management of the snail environment and human interactions with such areas, implementing surveillance of infection among livestock and poultry, improving stool treatment processes, and applying pharmaceutical interventions upon snail populations. Additionally, active and passive monitoring need to be combined to provide timely detection of local or imported schistosomiasis epidemics [24].

Conclusions

The risk of schistosomiasis transmission in this area still exists, and risk monitoring of schistosomiasis should be strengthened. Jiangsu Province remains in strict accordance with the National Schistosomiasis Monitoring Program (2014 version) requirements, and carefully carries out regular monitoring of schistosomiasis [36] with the goal of providing timely detection and treatment in the event of an epidemic, and strive to for early detection, early treatment, and early control. Jiangsu Province has seen much success in the control of schistosomiasis across the province [37]. However, there remains the need to further strengthen the monitoring of schistosomiasis in the region in order to fully actualize the goal of schistosomiasis prevention and elimination.

Abbreviations

CI

Confidence interval

ELISA: 

Enzyme-linked immunosorbent assay

OD: 

Optical density

Declarations

Acknowledgements

The author acknowledges the support of collaborators and partners from the Dantu Center for Disease Control and Prevention and ZhengJiang Center for Disease Control and Prevention.

Funding

This project was supported by the National S & T Major Program (grant no. 2012ZX10004220), Project of National Natural Science Foundation of China (No. 81101275) and Capacity improvement project of Jiangsu Public Welfare Institute (No. BM2015024).

Availability of data and materials

Data sharing is not applicable as no datasets were generated or analysed in this paper.

Authors’ contributions

KY and XYW conceived of the study, carried out the realist review, wrote the first draft of the paper and finalized the paper. JX, SZ, WL, JFZ and AS provided input into study design, contributed to the first draft of the paper and helped finalize the paper. All authors read and approved the final manuscript.

Ethics approval and consent to participate

All studies described here were approved by the Ethics Review Committee of Jiangsu Institute of Parasitic Diseases, China (Permission number: JIPDERC2010008). The field studies did not involve endangered or protected species.

Competing interests

The authors declare that they have no competing interests.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Jiangsu Institute of Parasitic Diseases, Yangxiang117#, Meiyuan, Wuxi, 214064, Jiangsu, China
(2)
Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health; Jiangsu Provincial Key Laboratory on the Molecular Biology of Parasites, Yangxiang117#, Meiyuan, Wuxi, 214064, Jiangsu, China
(3)
Public Health Research Center, Jiangnan University, Jiangsu, Sheng, China
(4)
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China

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