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National prevalence and risk factors for tungiasis in Kenya



Tungiasis is a highly neglected tropical skin disease caused by the sand flea, Tunga penetrans, the female of which burrows into the skin, causing pain and itching. The disease occurs throughout South America and sub-Saharan Africa but there are few systematic data on national disease burdens. The tungiasis research community is keen to develop survey methods to fill this gap. Here we used a school-based, thorough examination method to determine the prevalence and risk factors for tungiasis in Kenya.


We conducted the first nationally representative survey of tungiasis, including nine counties covering the major ecological zones of Kenya. A stratified multistage random sampling was used to select 22 primary schools from each of the nine counties and to select up to 114 pupils aged 8 to 14 years in each school. Pupils were examined thoroughly for tungiasis. Two surveys were conducted, the first between May and July 2021 and the second between October 2021 and April 2023 when pupils were also interviewed for risk factors. Mixed effect logistic regression models were used to test associations of independent variables with tungiasis using the school as a random effect.


The overall prevalence of tungiasis in the first survey was 1.35% [95% confidence interval (CI): 1.15–1.59%], and 0.89% in the second survey. The prevalence ranged from 0.08% (95% CI: 0.01–0.59%) in Taita Taveta county to 3.24% (95% CI: 2.35–4.44%) in Kajiado county. Tungiasis infection was associated with county of residence, male sex [adjusted odds ratio (aOR) = 2.01, 95% CI: 1.52–2.67], and lower age (aOR = 0.81, 95% CI: 0.75–0.88). For the first time we demonstrate an association with attending public schools rather than private schools (aOR = 5.62, 95% CI: 1.20–26.22) and lower socioeconomic status (aOR = 0.10, 95% CI: 0.03–0.33). Using a rapid screening method of the top of feet only, would have missed 62.9% of all cases, 78.9% of mild cases and 20.0% of severe cases.


Tungiasis is widely but heterogeneously distributed across Kenya. School-based surveys offer an efficient strategy for mapping tungiasis distribution.

Graphical Abstract


Tungiasis is a skin disease caused by adult female sand fleas, which burrows into the skin, mostly of the feet. Once embedded, the female grows 2000-fold over 7 days as eggs develop in the abdomen causing inflammation, and pain and itching which in turn cause difficulty in walking, sleeping and concentrating.

The disease mostly affects children, the elderly and disabled people in resource-poor, marginalized populations in Central and South America and sub-Saharan Africa [1]. The main risk factors for tungiasis have been identified from multiple studies in resource-poor communities having a high prevalence of tungiasis. The factors are all poverty-related including living in a house with an unsealed earthen floor [2,3,4,5,6], ownership of pigs or dogs [2, 7,8,9], not wearing shoes [7, 9, 10] and not always washing feet with soap [3,4,5].

In 2020, tungiasis was added to the World Health Organization’s (WHO) Roadmap for Neglected Tropical Diseases (NTDs) under scabies and other “ectoparasitoses” and is targeted for control [11]. Individual surveys have demonstrated prevalence to range from 7% in a village in coastal Kenya [12] to 62.8% in Napak District in northeastern Uganda [13], but there are no systematic data on the overall disease burden and distribution in any one endemic country. This information is critical to plan the scale of and to target interventions to the right communities.

As countries prepare to assess their disease burden, discussions are being had as to how best to do this, using village or school-based surveys, online forms, or mobile apps to be used by health workers, community health volunteers or non-governmental organisations. Full examinations are laborious, and new protocols have been proposed using rapid observations. Unlike many other skin diseases, tungiasis is quite distinctive in appearance making it relatively simple to diagnose and unlikely to be confused with another skin disease. One option which has been proposed in the literature is a rapid screening technique, lining school pupils up and quickly observing the top of their feet for periungual lesions [14]. This method has not been subjected to a rigorous comparison with full examinations in a large population with a dark skin.

In this study, we aimed to determine the national prevalence of tungiasis in Kenya, to assess whether a rapid survey method would be sufficient for future surveys, assess disease severity and to determine explanatory factors for infection to identify possible interventions that could reduce the disease prevalence.


Study design

This study consisted of two cross-sectional surveys of primary school pupils with the first survey conducted between April and June 2021 which is the main wet season in most counties. The second survey was conducted in a different group of randomly selected schools from September 2021 to April 2023 but varying in duration from county to county and spanning dry and wet seasons (Table 2). The surveys focused on pupils aged 8 to 14 years since this is the age group previously established to be most affected [2, 12] and able to answer questions about their home and family.

Study implementation

To build capacity for tungiasis surveillance, the surveys were conducted by partnering in each county with the Departments of Health and Education. In Kenya management of health services is devolved to the 47 counties which is managed through a County Health Management Team (CHMT) headed by a county executive, a director and chief officer. The Department of Health nominated a CHMT member, usually the County Disease Surveillance Officer or NTD Coordinator, to coordinate the study and recruit a team of five people to carry out the surveys. The principal investigator reviewed curricula vitae of potential team members who were mostly public health graduates. The principal investigator trained all team members and the coordinator. In every school the public health officer responsible for the area joined the team and two community health volunteers were trained to assist. The Department of Education gave approval for the study and provided school lists for each sub-county.

Sample size

The sample size for assessing tungiasis prevalence in Kenya was calculated using a simple random sample formula [15] taking into account the design effect due to clustering [16], the stratified sampling approach, and available resources. The main outcome was tungiasis prevalence in Kenya. Previous studies have reported prevalence of tungiasis to range from 15–60% within schools, thus we assumed an average prevalence of 30%. For a study with 95% confidence levels (Zα/2 = 1.96), 95% precision, a cluster size of 110 and assuming an intraclass correlation coefficient (ICC) of 0.125 (based on calculations for soil-transmitted helminths), we estimated a sample size of 11,010 pupils spread across the 5 ecological zones (2 counties in each). If we maintained a cluster size of 110, cluster being one primary school, we would need 20 schools in each climate zone. Since one county declined to participate the sample size of 11,010 was then re-distributed across 9 counties requiring 11 schools per county and 114 pupils were targeted in each school.

Study population

Nine counties of the 47 in Kenya were purposively selected to represent the main climatic zones. These included: Kericho and Muranga as humid; Nakuru as semi-humid; Kajiado, Kilifi and Makueni as semi-arid; Taita-Taveta and Samburu as being mostly arid; and Turkana as very arid (Fig. 1).

Fig. 1
figure 1

Rainfall map of Kenya and counties enrolled in the study (bold type). Map generated by C. Nyundo, KEMRI-Wellcome Trust Research Programme in ArcGIS 10.8.2 using rainfall data from Kenya Meteorological department website

Stratified random sampling was used to select 22 primary schools in each participating county ensuring coverage of all sub-counties, using lists provided by the County Department of Education. Within each selected school an equal number of boys and girls were quasi-randomly selected by asking the pupils to assemble in three age groups; 8 and 9 years; 10 and 11 years; 12 to 14 years, and by sex within each age group. For each of these age/sex groups, every nth pupil (n = total number in the group/19) was selected until 19 was selected, giving a total of 114 overall.

Clinical assessment procedures

The feet of the 114 children in each participating school were washed and dried and systematically examined by trained field workers for the presence of tungiasis as described previously [17]. Those pupils found to have fleas in their feet, were assessed for intensity of infection by counting the number of embedded fleas (live, dead, manipulated lesions and flea clusters). For infected pupils, field officers also recorded whether the infection could be detected by simply looking at the top of the feet (peri-ungual areas) alone.

Infected pupils were also examined for associated morbidity recording the presence of symptoms that are easily identified by non-clinical field officers; desquamation, fissures, ulcers and abscess for acute symptoms and hyperkeratosis, deformed nails and lost nails for chronic symptoms. Symptoms normally recorded by previous studies [18] but omitted here were oedema, erythema, warmness and peri-ungual hyperkeratosis. All pupils were also observed for any obvious disability and signs of other skin diseases or abnormalities.

Explanatory variables

All schools were categorized either as public or private during the survey and further as urban or rural based on housing density in their location. In the second survey, six pupils without infection were randomly selected in each school using the paper lottery method and were invited for interview together with all infected pupils. An opt-out informed consent process was used as described further in the Ethics section below. All selected pupils were interviewed using a structured questionnaire collecting information on demographic, behavioural, social, parental, and economic factors.

Data analysis

All analyses were conducted in Stata IC version 15.1 (Stata Corp LLC, College Station, Texas, USA). Figure S1 in Additional materials (Additional file 1), illustrates the pupil selection and numbers included for each analysis. The prevalence of tungiasis (proportion of examined participants infected with tungiasis in their feet or hands) and 95% confidence intervals (CIs) were calculated for the country as a whole, for each county and for each school based on data collected from the first cross-sectional surveys only. A chi-squared test was used to test for a significant difference between counties. The second round of cross-sectional surveys in the counties was conducted over a period of one and a half years due to logistical challenges and therefore data could not be used to obtain a point prevalence.

For analysis of infection intensity and associated symptoms, a new dataset was created merging all infected participants from both surveys. The infection intensity for each infected individual was calculated as the sum of all flea stages (live, dead and manipulated) on both feet, plus the number of flea clusters multiplied by 5 (as an estimate for an average number of fleas/cluster). A total clinical score was calculated for each patient by summing the number of areas on the feet (each foot being divided in to 9 areas: 5 toes, medial side, lateral side, heel and sole) exhibiting acute symptoms (desquamation, fissures, ulcers and abscess) to a maximum score of 72, and chronic symptoms (hyperkeratosis, deformed nails and lost nails) to a maximum score of 38, to give an overall maximum clinical score of 110.

A scatter plot was created of infection intensity by clinical score for all infected cases and a polynomial regression line fitted. Patient disease severity was then classified using a recently developed threshold [17] of 10 embedded fleas, less than 11 fleas being considered mild disease and more than 10 fleas being severe disease. To determine the relationship between county prevalence and disease severity a scatterplot and fitted linear regression was created of the number of cases in each county from both surveys combined and the percent of those cases that were severe.

To test for associations between tungiasis infection status (infected and not infected) and possible explanatory variables, two-level mixed effects logistic models were used with an exchangeable correlation matrix and school ID number used as random effect. Sex of the pupil, school class, age at survey, disability status, having other skin disease(s), school type (public/private), school location (urban/rural), socio-economic status (SES) of the pupil, and county were included as fixed effects. County was not considered random because (1) their number was small, and (2) they were not selected at random. Initially, univariable analyses were run for each explanatory variable and then those with a P-value less than 0.2 were included in the multivariable model. Backward elimination, sequentially excluding variables was used to develop the final model using Akaike information criteria (AIC) to compare the models. Models with lower AIC values were preferred. Additional consideration on variable selection was expert knowledge on potential relationships with the outcome. Interactions terms were explored but were not significant and were therefore not included in the models. Wald tests were also run for validity of variables included in the final model and are presented as p-values in the footnote to the table. Univariable effects were presented as odds ratios (OR) and multivariable effects as adjusted odds ratios (aOR). The SES variable was derived through polychoric principal component analysis as described in Additional material S5 (Additional file 1).

The intra-cluster correlation coefficient (ICC) was calculated for the full data set with 21,467 pupils to guide future study designs and sample size calculations and to assess the need for a multilevel analysis. The ICC measures the relatedness of measurements from pupils within a school and ranges from 0 (pupils within a school are as heterogeneous as pupils between schools) to 1 (pupils within a school show identical measurements) [19].


During the first cross-sectional surveys between May and September 2021, 99 schools were surveyed with a total of 10,865 pupils examined for tungiasis (Table 1). In the survey between October 2021 and April 2023, 97 schools were surveyed with a total of 10,600 pupils examined.

Table 1 Characteristics of the study population

Prevalence of tungiasis

The overall prevalence of tungiasis during the first survey was 1.35% (95% CI: 1.15–1.59%, Table 2). Of the infected pupils, 7 (4.8%) had fleas only in the hands but not in their feet. The prevalence varied markedly between counties, from 0.08% (95% CI: 0.01–0.59%) in Taita Taveta to 3.24% (95% CI: 2.35–4.44%) in Kajiado. During the second survey only 0.89% of pupils were found infected (Table 2).

Table 2 Prevalence of tungiasis by county and survey

The prevalence also varied considerably across schools, with 41 of 99 schools (41.4%) in the first survey, and 35 of 97 schools (36.1%) in the second survey, having at least one case (Table 2). The majority of the affected schools (82.9%) having a prevalence of less than 5%, and only 3 schools having a prevalence of more than 10% (Table 2, seen in survey 1). There was heterogeneity within county: for instance, in Kajiado and Samburu counties most of the cases (67.6% and 68.4% of all cases in the county, respectively) were recorded from only one of eleven schools. On the other hand, in Muranga county, the disease was more evenly distributed with infected pupils identified in seven schools with only one school having more than five cases.

Disease severity

To assess intensity of infection and morbidity, data on all infected pupils were combined for both surveys (n = 242). The extent of symptoms (clinical score) correlated strongly with the infection intensity (R2 = 0.80, S2 in Additional file 1). The recently developed two-tier disease severity threshold of 10 embedded fleas [17] aligned well with our data, clearly separating the majority of cases with less than 11 fleas and presenting with low clinical scores from those pupils with more than 10 fleas and higher clinical scores, hence severe disease.

The percent of infected pupils with severe disease in each county was correlated to the overall number of pupils infected in both surveys (R2 = 0.7, S3 in supplementary materials, Additional file 1) with the exception of Nakuru county (red dot in S3) where despite a relatively high number of cases (38) only 2.6% of the cases were severe (Table 3).

Table 3 Number of pupils examined, and percent infected for each affected school by county and survey

Evaluating rapid disease assessment

Observing only the tops of unwashed feet has been suggested as a rapid method for national surveys to assess tungiasis prevalence. However, of the 221 infected pupils for whom this was tested, only 82 (37.1%) had an infection that could be detected on the top of their feet. Using this rapid screening method would have missed 62.9% (n = 139) of all cases, 78.9% (n = 127) of 161 mild cases and 20.0% (n = 12) of 60 severe cases.

Explanatory variables associated with tungiasis

Univariable analysis of data from the entire study population of examined pupils suggested that infection was associated with the county of residence, the school type, the class, age, sex and other skin conditions (Table 4). All of these variables were taken forward to the multivariable model except survey round and location type (urban/rural) as they were not associated with infection. The multivariable model highlighted the heterogeneity in disease prevalence between counties (Table 4) and demonstrated boys were twice as likely to be infected than girls. Children in public schools were five times as likely to be infected as those attending private schools (aOR = 5.62, 95% CI: 1.20–26.22). There was also a strong association between tungiasis infection and the presence of other skin conditions in examined pupils (aOR = 3.60, 95% CI: 2.38–5.45). There was no association of tungiasis infection with rural or urban location of the school (OR = 1.15, 95% CI: 0.49–2.70).

Table 4 Risk Factors for tungiasis in the whole pupil population (n = 21,246)

The estimated intra-cluster correlation coefficient for tungiasis was 0.40 (95% CI: 0.37–0.58) indicating a stronger correlation between individuals in a cluster than between clusters, which in this study were the schools.

Detailed interviews were held with a subsample of cases, including 330 boys (51%) and 313 girls (49%) with a mean age of 11.1 years (SD = 1.96). Only nine (1.4%) pupils in this group had a disability and 38 (5.9%) had another skin abnormality. Of the 643 interviewees, 77 (11.9%, 95% CI: 9.6–14.7%) were infected (S4 in Additional material, Additional file 1).

Univariable analyses identified several factors associated with tungiasis infection (Table 5). Variables that were significant predictors (P < 0.200) were taken forward to a multivariable model and retained in the multivariable model where P < 0.050 (Table 5). County and socio-economic status remained associated with tungiasis. Children who said they never use soap for foot washing had a nearly six times higher (aOR = 5.80, 95% CI: 1.08–31.17) odds of infection than those who say they always use soap. Pupils who say they wash their feet less than once a day had four times higher odds of infection (aOR = 4.64, 95% CI: 1.16–18.54) than those who said they wash more than once a day. Although not having a significant P-value, living in a house with floors of loose sand or soil was associated with infection (aOR = 2.57, 95% CI: 0.99–6.70) since dropping this variable from the model resulted in the AIC increasing (Table 5).

Table 5 Risk factors from the selected pupils in the second survey (n = 643)


Countries with endemic tungiasis struggle to design and target appropriate interventions due to the absence of data on country-wide disease burden, geographic distribution and characteristics of target communities. A number of small-scale studies that were specifically targeted in high burden settings [3, 10, 12] have shown that tungiasis can represent a major challenge for the primary health care system in Kenya. However, to establish data-informed guidelines and interventions, we needed to systematically assess the national disease burden of tungiasis in Kenya. To our knowledge, this is the first survey of this scale for any endemic country. We demonstrated that the national prevalence during the major rainy season in 2021 was 1.3%.

This is similar to another survey conducted in all households in the Kwale health and demographic surveillance system in 2011 [20], which found a prevalence of 1.1%. Other prevalence surveys that have been published to date report a wide range of prevalence from 7% in a village in coastal Kenya [12] to 63% in Napak district in northeastern Uganda [13]. These surveys did not randomly select clusters, but specifically targeted schools or villages previously known to have high numbers of cases. In addition, they were also mostly conducted during the dry season which has been associated with a higher prevalence in Brazil [10, 13, 21].

What is striking from the current survey is the high percentage (41%) of schools that have at least one case, indicating the disease is widespread across the country. The large difference in prevalence between counties and even between schools in the same county reflects the extreme heterogeneity in the distribution of tungiasis [3, 12, 22] which poses a considerable challenge for planning and targeting interventions.

Recently a new two-tier classification of mild versus severe disease was proposed [17], using the correlation of infection intensity with clinical signs. We applied this to the current population and confirmed a good fit of the proposed cut off point of 10 fleas as a suitable threshold to separate cases with low and high levels of morbidity. This classification revealed the proportion of cases with severe disease correlates with the number of cases in a county, and by inference, prevalence of disease. The unusually low proportion of cases with severe disease in Nakuru could be the result of past interventions in the county (County Health Management Team personal communication). When treatment is always available in a community, people can obtain treatment as soon as they are newly reinfected and therefore will not accrue a high intensity of infection, as seen during a community-based effort to control tungiasis in Kenya [23].

We assessed SES from our interview data and demonstrated a very strong association. Living in poverty exposes people to other risk factors such as living in a house with an unsealed earthen floor, and/or lacking access to water and soap. The association with attending a public school rather than a private one is probably also related to SES, but in addition, private schools may have better access to water and the teachers and parents pay more attention to the personal hygiene of pupils. All possible explanations that require further investigation. Likewise, the association with other skin abnormalities is likely to be linked to SES, for example, through shared risk for scabies, head lice and/or fungal infections [24].

The possible protective role and mechanism of at least daily foot washing with soap needs further investigation, as does the higher odds of infection for boys, which is a common finding in all tungiasis surveys. Risk in boys could be due to behaviours that put them more at risk of infection, differing behaviour of caregivers, or as yet unknown biological factors.

Anecdotal reports suggest people with disabilities, either physical or mental, living in endemic areas have a high infection intensity. In the current study we attempted to identify pupils with disabilities without the use of a formal tool. Our data seem to support this, but the small sample size resulted in a wide confidence interval, hence further research should specifically investigate the risk associated with disability.

One limitation of this survey was the low number of infected cases available to enrol for the risk factor interviews that resulted in the large confidence intervals. A second limitation was the protracted time it took for all counties to complete the second survey which meant we could not obtain a second prevalence estimate in a different season. The delay in some counties was caused by delays in mobilising funds for the team which were exacerbated by campaigns and national elections in 2022. Another limitation could have been the use of school-based surveys which could miss children from the poorest families who cannot afford the costs to attend school, or those with such severe disease they were unable to walk to school due to the pain. Nevertheless, our surveys did include children with low SES as assessed here (10% of the interviewed pupils) and 26% of infected pupils had severe disease. Consequently, we suggest a public school-based strategy is likely to be a scalable and cost-effective means for mapping infected communities.

The process of washing, drying and thorough examination of feet is time consuming, but we have demonstrated that replacing it with a quick look at the top of feet alone, as suggested previously [14] would miss two thirds of infected children in this population. Instead, we would recommend further research to test a method that would quickly look at both the bottom and top of the feet, only washing those that are very dirty. This could be conducted by trained teachers or community health workers linked to the school.


Findings from this survey confirm our previous observations that tungiasis could be reduced through a one health approach addressing treatment, control of off-host stages in unsealed earthen house floors and improved personal hygiene. Animal interventions may be required in some communities, but neither the current study nor previous ones in Kenya have demonstrated a role for animals in transmission in Kenya. Currently large-scale WASH (water, sanitation and hygiene) programs are conducted globally focussing entirely on handwashing, but if this messaging could change to whole body washing with soap, they could address multiple diseases, including tungiasis. Interventions retrofitting low-cost floors into houses of infected families need to be explored and their efficacy compared with WASH interventions which may be more affordable but have a short-term impact. The link with poverty is typical for many infectious diseases and re-emphasizes the need for integrated and community-based development and disease control programs.

Availability of data and materials

The datasets and other materials supporting the conclusions of this article are available on KWTRP Research Data Repository at Harvard Dataverse through the following link:


aOR :

Adjusted odds ratio


County Health Management Team

CI :

Confidence interval


Kenya Medical Research Institute


Neglected tropical diseases

OR :

Odds ratio


Parent Teachers Association


Socio-economic status

SD :

Standard deviation


Water, sanitation and hygiene


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We are grateful to the County Tungiasis Team Leaders: Wambani Zablon3, Collins Kipkorir4, Philip Nzyoka5, Moses Obiero6, Marawan Elijah7, Jacob Kapombe8, Kuyan Malano9, Caleb Kasuku10, Rael Kukule11 from Departments of Health in 3Muranga, 4Kericho, 5Makueni, 6Nakuru, 7Samburu, 8Kilifi, 9Kajiado, 10Taita Taveta, 11Turkana, We are also grateful to the communities who participated, the school Parent Teacher Associations and Head Teachers who allowed us to work in their schools, the county Directors of Health and Education who gave their approval for the study and assisted in many ways to ensure its success. We acknowledge the efforts and dedication of Henry Kivuva (RIP) to set up the survey in Makueni County.


Research funding for this work was provided by the Wellcome Trust through the project “Epidemiology of Tungiasis” (grant number 213724/Z/18/) granted to Lynne Elson as a Career Re-Entry Fellowship. This work was written with the permission of Director KEMRI-CGMRC. The views expressed herein do not necessarily reflect the official opinion of the donors. The funders had neither a role in the design of the study, nor in collection, analysis, interpretation of data nor in writing the manuscript.

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Authors and Affiliations



Conceptualization, LE, UF, MM, IA, PB; Methodology, LE, UF, PB, BO, MM, IA; Formal Analysis, LE, BO; Investigation, LE, CK, SK, CM, MO, ES, JK, EM, MW, JL; Resources, LE, PB; Data Curation, LE ; Writing—Original Draft Preparation, LE.; Writing—Review & Editing, UF,BO, PB, MM, IA; Visualization, LE.; Supervision, PB; Project Administration, LE, PB, MM; Funding Acquisition, LE, MM, PB.

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Correspondence to Lynne Elson.

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Ethics approval and consent to participate

The study was approved by the KEMRI Scientific and Ethics Review Committee (approval number KEMRI/SERU/CGMR-C/170/3895) as well as the Oxford Tropical Research Ethics Committee (reference number 38-19). During the community engagement phase, a presentation was made to the national Director for Neglected Tropical Diseases at the Ministry of Health, the county and sub-County Health Management Teams and the Department of Education in all counties to obtain their approval. In each school a meeting was held with the school Parent Teachers’ Association (PTA) or management board to obtain their permission to conduct the survey in their school. The head teacher and PTA chairperson signed the consent form on behalf of the parents and school for the pupils to be examined. Each child gave verbal assent. Community health workers were hired and trained in each school to assist and be the link with the community emphasizing that participation was completely voluntary, and subjects had the opportunity to withdraw from the study at any point in the study. For pupils selected for interviews, these were explained, and each was given an information leaflet to take home for their parents along with an opt-out form. Parents were to sign and return the form only if they did not want their child to participate in the interviews, or they could attend the school the next day to clarify any issues they may have. On the following day, if the selected pupils did not have the opt out form and were willing to participate, they proceeded with the interviews. All data were collected on PIN protected electronic tablets, stored on password protected RedCap databases on the KEMRI-Wellcome Trust servers. Data were analyzed after export to Excel spreadsheets without inclusion of personal identifiers. All pupils with tungiasis were referred for treatment to the community health workers or the local health facility using benzyl benzoate provided by the study. For those with secondary bacterial infection and other illnesses requiring treatment, a referral was made to the nearest health facility.

Consent for publication

Not applicable.

Competing interests

All authors declare no conflict of interest.

Supplementary Information

Additional file 1: S1:

Flow diagram describing selection of participant groups. Orange boxes represent pupils infected with tungiasis; green boxes represent uninfected pupils. Blue arrows depict flow in the first survey, orange arrows depict flow in the second survey. S2 Correlation of infection intensity with clinical score for all pupils with tungiasis in their feet (n = 242) with a fitted linear regression line (dotted blue line). The red line indicates the 10-flea threshold for mild and severe disease. S3 Correlation of county percent infected with percent of cases with severe disease (n = 225). S4 Demographics of pupils selected for risk factor interviews. S5 Polychoric Principal Component Analysis for a Socio-Economic Variable, Cronbach alpha and correlation with county poverty rate.

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Elson, L., Kamau, C., Koech, S. et al. National prevalence and risk factors for tungiasis in Kenya. Infect Dis Poverty 12, 85 (2023).

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  • Tungiasis
  • Neglected tropical diseases
  • Prevalence
  • Disease burden
  • Kenya