- Research Article
- Open Access
Prevalence and risk factors of schistosomiasis and soil-transmitted helminthiases among preschool aged children (1–5 years) in rural KwaZulu-Natal, South Africa: a cross-sectional study
Infectious Diseases of Povertyvolume 8, Article number: 47 (2019)
Despite efforts to control neglected tropical diseases (NTDs), schistosomiasis and soil-transmitted helminthiases remain widely prevalent in sub-Saharan Africa. Recent data suggest that these infections are prevalent among preschool aged children (PSAC) in poor communities. Evidence of schistosomiasis and soil-transmitted helminths (STH) infection patterns and prevalence among PSAC is essential for effective treatment and control programmes. The aim of the study was to determine the prevalence, intensity and risk factors of schistosomiasis and STH infection among PSAC in the Ingwavuma area of uMkhanyakude District, South Africa.
A cross-sectional study was conducted among 1143 PSAC aged 1–5 years in 34 preschools and early childhood development (ECD) centres. Data on risk factors was collected using a semi-structured questionnaire. A Kruskal–Wallis test was used to compare the differences in infection intensity with age. Pearson Chi-square test and multivariate logistic regression were performed to assess the association between PSAC infection status, sociodemographic, household, water and sanitation variables and hygiene practices of PSAC and their caregivers.
We observed a low prevalence of Schistosoma haematobium (1.0%) and S. mansoni (0.9%). The prevalence of Ascaris lumbricoides (18.3%) was high compared to Trichuris trichiura (1.2%), hookworms (1.6%) and Taenia (6.4%). The odds of schistosome infection were lowest among PSAC under younger (15–24 years) caregivers (0.1, 95% CI: 0.02–0.54) and those who used tap water (0.3, 95% CI: 0.09–0.78) for domestic purposes. Schistosome infection was however higher among PSAC who bathed in river water (17.4, 95% CI: 5.96–51.04). STH infection on the other hand was lowest among PSAC who did not play in soil (0.1, 95% CI: 0.51–0.28), were from households that used tap water for domestic purposes (0.5, 95% CI: 0.27–0.80) and PSAC under the care of younger (25–35 years) caregivers (0.3, 95% CI: 0.10–0.75). The risk of STH infection was highest among PSAC who did not wash their hands with soap (3.5, 95% CI: 1.04–11.67) and PSAC whose nails were not trimmed (3.6, 95% CI: 1.75–7.26).
The findings show low prevalence and infection intensity of schistosomiasis and STH infection except A. lumbricoides among PSAC. Factors predicting schistosomiasis and STH infection among PSAC were related to caregivers’ age, educational status, water and hygiene practices. STH infection was exclusively associated with PSAC playing and handwashing habits. These findings highlight the need to include PSAC caregivers in schistosomiasis and STH prevention and control programmes.
Please see Additional file 1 for translations of the abstract into the five official working languages of the United Nations.
Schistosomiasis, also known as bilharzia and soil-transmitted helminths (STH, Ascaris lumbricoides [roundworms], Trichuris trichiura [whipworms], and hookworms) are the most prevalent parasitic infections worldwide . Global estimates suggest that schistosomes infect about 230 million people while A. lumbricoides, T. trichuris and hookworms infect 1.221 billion, 795 million and 740 million people, respectively . Ninety per cent of the people infected worldwide reside in sub-Saharan Africa (SSA). Due to the overlap in their geographical distributions , schistosomiasis and soil-transmitted helminthiases can occur independently or jointly depending on the nature of risk factors and host related attributes [1,2,3,4]. Risk factors such as access to safe water and sanitary related practices of caregivers and children have been reported to be associated with STH infection [5, 6]. Whilst school aged children (SAC) acquire schistosomiasis by participating in risky water practices [7, 8], preschool aged children (PSAC) are passively exposed to infection when their parents perform daily water-related chores [9, 10].
PSAC may be exposed to infection within the first 5 years of life and thus contribute to the observed heavy burden of infection in the SAC. The burden associated with schistosomiasis and STH infection exhibits notable commonalities; (i) both infections thrive in poverty-stricken areas with limited or no access to safe water supply and basic sanitation [3, 11], (ii) pathology is related to worm burden and may lead to death in heavily infected individuals, (iii) they retard the physical and cognitive development of children and hinder educational advancement [12,13,14], (iv) schistosomes and STH-immunosuppressive features may predispose infected individuals to HIV/AIDS [15,16,17], and (v) chronic infection may cause severe illness and irreversible disabilities such as cancers of the bladder, prostate, kidney, liver or intestines [18,19,20] .
In South Africa, schistosomiasis and STH infection are more prevalent amongst disadvantaged children who live in poor rural communities. High levels of infection have been documented amongst children in most provinces in South Africa, including KwaZulu-Natal [7, 21], Mpumalanga , Port Elizabeth  and Limpopo . South Africa is a signatory to the World Health Assembly (WHA) resolution 54.19 , which calls for regular treatment of worms in high-risk groups. Mass drug administration for the treatment of soil-transmitted helminthiases is currently rolled out in primary schools. However, the mass treatment for schistosomiasis treatment has not yet been initiated. In South Africa about 5.2 million people need annual preventive chemotherapy against schistosomiasis per year, mostly targeting SAC . Schistosomiasis and soil-transmitted helminthiases control measures include the provision of water and sanitation, health education, snail control and treatment [27, 28]. Currently, control programmes mainly focus on the treatment aspect and yet studies have shown that treatment-based control programmes have a temporal effect on transmission . It is for this reason that the World Health Organization (WHO) expects endemic countries to develop comprehensive strategies aiming at eliminating soil-transmitted helminthiases and schistosomiasis amongst other NTDs by 2020 [3, 27]. This move demands urgent and aggressive programmes designed to both control morbidity and curtail the spread of infection. Endemic countries are still grappling with the search for the most important factors responsible for the rapid spread of soil-transmitted helminthiases and schistosomiasis. The knowledge of contextual risk factors may assist programme managers in channelling resources where it matters. Our study is thus timely and pertinent considering the shift from morbidity control to elimination of schistosomiasis and soil-transmitted helminthiases in many endemic countries. The objective of this study was to determine the prevalence and risk factors of schistosomiasis and STH infection among PSAC aged 1–5 years in the Ingwavuma area of uMkhanyakude District, South Africa.
Study area and population
This study was conducted in Ingwavuma rural community located in the northern part of uMkhanyakude (see Fig. 1, source: Manyangadze, Chimbari ), the third-poorest district in KwaZulu-Natal, South Africa . Ingwavuma area, our study site is located within the Jozini local municipality which covers about 32% (3057 km2) of uMkhanyakude District . Jozini has a total population of 186 502 people of which 89% reside in the rural areas. Most of the population (99.2%) are black Africans of which 94.6% are Zulu speakers. The Jozini municipality has a total of 20 wards. Our study was carried out in three wards covering 582 km2 and a population of 28 384 people . The unemployment rate in the district is 72.1%. People in this area live under the jurisdiction of traditional leadership and have limited access to basic services. For instance, 60% of the population lacks access to electricity, 38.2% have no access to tap water, whilst 18.2% have no access to toilets .
The area is arid with seasonal dams and characterized by hot and humid summer with a cooler and drier winter season. Multiple hills within the area improve water drainage, the Pongola and Ingwavuma rivers serve as the major drainage channels and are major sources of irrigation water . Except for a minority of households with access to tapped water, most households rely on rivers, seasonal streams, dams and ponds for gardening and laundry. However, drought conditions in the study area from 2014 to 2017 dried out most water bodies . uMkhanyakude District is formed of the following three related vegetation types: (i) mixed compound leaved short woodland (trees between 5 and 10 m) and wooded grasslands; (ii) open bushveld with dominant Acacia and Combretum species and Themeda triandra grass being the dominant grass in undisturbed areas; and (iii) open, tall, sour, wiry grasslands, often dotted with low bushes and solitary savannah trees. Most of the area is covered by sandy soil, particularly in the Maputaland region. The rest of the area is covered by Namib soil which is deep, acidic and well-drained; however, clay soil is found in some few areas [35, 36].
Study design and enrolment of participants
This was a cross-sectional study in which 1143 PSAC participated (see Fig. 2). Prevalence of schistosomiasis and STH infection among PSAC in the study area is unknown. This study was part of a bigger ongoing study that sought to screen all PSAC in accessible early child development (ECD) centres and pre-schools in the area whose caregivers consented to the study. Consent forms were distributed to 1600 PSAC but only 1462 returned signed consent forms and were eligible to participate in the study. The participants were drawn from 21 ECD centres and 13 preschools from 14 main villages within the Ingwavuma area. In the South African public education system, preschools are commonly known as grade R or grade 0 and are found within primary schools whilst ECD centres mostly standalone. Therefore, all primary schools within wards 15, 16 and 17 in our study area were eligible for inclusion in the study and were therefore selected. Furthermore, registered ECD centres around the selected primary schools were included in the study. During sample collection visits, 319 children could not provide any specimen and were therefore excluded from the study. Our study sample comprised of 1143 PSAC who provided urine samples out of whom 998 provided stool samples. Caregivers (parents and guardians) of children enrolled in the study responded to a schistosomiasis and soil-transmitted helminthiases risk factor questionnaire. Caregivers were eligible to participate if (1) they were present in schools and ECD centres during the day of screening, (2) their children had provided urine and stool specimens; and (3) were willing to participate.
Urine and stool sample collection
The study was conducted from June to September 2018. Urine and stool samples were collected using baby potties. Samples were collected between 10:00 am and 2:00 pm for optimal egg count necessary for the diagnosis of schistosomiasis and STH infection . Caregivers of PSAC were oriented on specimen collection procedures prior to specimen collection. Potties and specimen bottles were marked with identification codes representing each child and distributed to project research assistants and caregivers of PSAC. The identification codes on specimen bottles were matched with caregiver questionnaire identification codes such that a caregiver shared the same code with his or her child. Collected samples were enclosed in the coded specimen bottles, covered in wooden boxes placed in an area with shade to maintain sample quality and immediately transported to a processing site in the study area. Distant schools were 30 min away from the processing site and most samples were processed within two hours of collection.
Urine samples were processed using the filtration technique  whilst stool samples were processed using the Kato-Katz technique . For the filtration, we used nucleopore membrane filters with a diameter 13 mm and pore size 12 μm. Each urine sample was well mixed, and 10 ml was filtered through the membrane filter which was then placed on a microscope slide. A drop of Lugol’s iodine was added and the slide was examined under a microscope using objective × 10 and × 40 by two experienced laboratory technicians. The number of S. haematobium eggs per 10 ml were counted and recorded for each child . For the Kato-Katz technique, each stool sample was pressed through a sieve to remove large particles. Part of the sieved stool was then transferred to the hole of a template on a slide. The hole was filled after which the template was removed and the remaining sieved sample (10 mg) was covered with cellophane which had been pre-soaked in glycerol. The preparation was pressed with a cover slip or a slide to become flat. The slide was then viewed, and eggs were counted, and the number of eggs per gram of stool (EPG) was calculated [37, 40]. Each slide was read independently by two technicians. S. haematobium infection was determined by the presence of eggs in 10 ml of urine while S. mansoni, and STH infection were determined by the presence of eggs in 1 g of stool.
The intensity of infection was classified into the three levels defined by WHO as light, moderate and heavy infection. The classification of thresholds on infection intensity varied between parasites; for S. haematobuim, < 50 eggs/10 ml was classified as light intensity, and > 50 eggs/10 ml was heavy intensity; for S. mansoni, < 100 EPG was light intensity, 100–400 EPG was moderate intensity, and > 400 EPG was heavy intensity . For A. lumbricoides, 1–4999 EPG was light intensity, 5000–49 999 EPG was moderate intensity, and > 50 000 EPG was heavy intensity; for T. trichiura, 1–999 EPG was light intensity, 1000–9999 EPG was moderate intensity, and > 10 000 EPG was heavy intensity; for hookworm, 1–1999 EPG was low intensity, 2000–3999 EPG was moderate intensity, and > 4000 EPG was heavy intensity . Unlike STH and schistosomiasis, it is not easy to determine the intensity of Taenia thus we only determined the prevalence of Taenia species. The difficulty is related to the fact that Taenia eggs are expelled in gravid proglottids thus it is difficult to count individual eggs. Each detached proglottid segment is estimated to contain about 50 000–60 000 fertile eggs [41,42,43].
Data were analyzed using SPSS version 25.0 computer software (IBM Corporation, New York, USA) after checking its completeness. The dependent variables were schistosomiasis and STH infection status among PSAC (where STH infection was taken to mean infection with A. lumbricoides, T. trichiura, and hookworm) and the intensity of infection. The prevalence and arithmetic mean intensity of infection among all children was examined with 95% confidence intervals (CIs). In estimating the prevalence and mean intensity of infection, children were stratified according to gender. Non-parametric one-way ANOVA (Kruskal–Wallis) was used to compare the differences in intensity of infection with age. Furthermore, Pearson Chi-square test was used to determine the differences in prevalence. Statistically significant variables in the bivariate analysis were used as predictors in the multivariate logistic regression. This was done for data relating to both children and caregiver to measure the strength of the association between the risk factors and infection status. The results were expressed as odds ratio (OR) with their 95% CI and statistical significance level, P < 0.05. Potential risk factors explored were demographic factors (sex, age, occupation and education level), water and sanitary related factors and behavioural factors associated with schistosomiasis and STH infection.
Overall, 1462 children were enrolled in the study but only 1143 gave urine samples of which 998 also gave stool samples. The sample comprised of 557 (48.7%) males and 586 (51.3%) females. The mean age of PSAC involved in the study was 3.94 (± 1.10 years) and their ages ranged between 1 and 5 years. The sociodemographic characteristics of the study participants are in Table 1.
Prevalence of schistosomiasis and soil-transmitted helminthiases
Of the 1143 PSAC who provided urine samples, 11 (0.1%) tested positive for S. haematobium. Of these, three cases were males while eight cases were females. On the other hand, of 998 PSAC who provided stool samples nine (0.9%) cases were S. mansoni infection; seven males and two females. A. lumbricoides, T. trichiura, hookworm and Taenia were diagnosed in the stool specimens analyzed. A. lumbricoides was the predominant intestinal helminth infection with a prevalence of 18.3% (95% CI: 13.9–18.3%) and the lowest STH infection was for T. trichiura which had a prevalence of 1.2% (95% CI: 0.54–1.83%) (Table 2).
Analysis of our data showed that the prevalence of A. lumbricoides increased with age (P < 0.001). Furthermore, the prevalence of Taenia was high among males (P < 0.012). There was no statistically significant difference between males and females in relation to other helminth infections (P > 0.05) (Table 3).
Coinfection and intensity of schistosomiasis and soil-transmitted helminthiases among PSAC
The arithmetic mean of infection intensity for S. haematobium was 30.40 eggs/10 ml (95% CI: 27.30–33.80) and it was 6.90 EPG (95% CI: 5.47–8.61) for S. mansoni. There were no significant differences in the intensity of S. haematobium (χ2 = 1.512, P = 0.824) and S. mansoni (χ2 = 0.122, P = 0.998) infection with age. Significant differences were observed in the number of infections per child with age (χ2 = 13.35, P = 0.0097) showing that the number of species harboured increased with age. The mean intensity of infection for A. lumbricoides was 2.17 EPG (95% CI: 1.74–2.61) while it was 0.56 EPG (95% CI: 0.369–0.747) for T. trichiuris. The prevalence of both mono and poly-infections increased with age, poly-infection was highest (27.8%) amongst 4-year-olds and least (0%) among 1-year-olds (Table 4). However, no significant difference was observed between poly infection status and gender (χ2 = 2.59, P = 0.107).
Risk factors associated with schistosomiasis in PSAC
In both bivariate and multivariate analysis, the caregiver’s age group, caregivers’ habit of bathing children in river water and source of domestic water supply were significantly associated with schistosome infection. The odds of schistosome infection were lower among PSAC with younger (15–24 years) caregivers (0.1, 95% CI: 0.02–0.54) and those who used tap water (0.3, 95% CI: 0.09–0.78) for domestic purposes. Schistosome infection was however higher among PSAC whose caregivers reported to be bathing them in river water (17.4, 95% CI: 5.96–51.04). (Table 5).
Risk factors associated with soil-transmitted helminthiases in PSAC
The prevalence of STH infection was significantly associated (P < 0.05) with the following factors: marital status, educational status, caregiver’s age group, type of toilet used at home, main source of domestic water, frequency of trimming child’s fingernails, caregiver’s handwashing habit after visiting the toilet, child’s habit of washing hands with soap and water and child’s habit of playing in soil (Table 6). In a multivariate logistic regression results presented in Table 5, the odds of STH infection were lowest among PSAC who did not play in soil (0.12, 95% CI: 0.51–0.28), those from households that used tap water for domestic purposes (0.47, 95% CI: 0.27–0.80) and PSAC under the care of caregivers within the age group 25–35 years (0.27, 95% CI: 0.10–0.75). The risk of STH infection was higher among PSAC who did not wash their hands with soap and water (3.49, 95% CI: 1.04–11.67), PSAC with primary level educated caregivers (4.16, 95% CI: 0.49–35.63) and those who did not have their nails trimmed (3.56, 95% CI: 1.75–7.26).
This study examined the prevalence, intensity and risk factors of schistosomiasis and soil-transmitted helminthiases among PSAC aged 1–5 years. To the best of our knowledge, this is the first study in South Africa that has attempted to examine schistosomiasis and soil-transmitted helminthiases among PSAC. Previous studies in South Africa have focused on schistosomiasis and or STH infection among SAC where the reported prevalence ranged from 24 to 80% [7, 16, 21, 22, 44, 45]. In our study, the prevalence of schistosomiasis was low. Our findings are similar to those for a study in Tanzania  that reported a 1.9% prevalence of S. haematobium among 424 PSAC who all had light infection. In contrast to our study, studies conducted in Mali , Zimbabwe , Uganda  and Nigeria [9, 10] reported a higher prevalence of schistosomiasis ranging between 21 and 86%. The relatively lower prevalence of schistosomiasis in our study may be related to the drought that affected uMkhanyakude, Ingwavuma area from 2014 to 2016 . Rainfall patterns changed considerably in the study area leaving most transmission sites dry. This also resulted in a decline in the prevalence of schistosomiasis among SAC within the study area . In addition, the administration of praziquantel to SAC within the study area since 2015  may have led to a reduction in infection rates. There is evidence that treating SAC infected with schistosomiasis tends to lower the prevalence among the entire population [51, 52].
In our study, PSAC gender and age were not significant predictors of schistosome infection. These findings are consistent with studies conducted in Tanzania , Nigeria  and Ethiopia . Furthermore, findings from the study done in Nigeria  also suggest that the gender and age of PSAC may not determine infection status among PSAC since schistosomiasis at this age is believed to be passively acquired depending on the caregiver’s exposure to open water sources. Nevertheless, the study showed that the risk of A. lumbricoides infection increased with age among the PSAC. The presence of poly-infection also increased with age of PSAC. This may suggest that those children acquire such infection as they become more independent and adventurous. Our findings corroborate with previous studies that showed age to be a predictor of STH infection among PSAC . The overall prevalence of STH infection was 20.6% with A. lumbricoides being the predominant parasite with a prevalence of 18.34%. A. lumbricoides infection was significantly associated with the age of the child but not with gender. According to the WHO classification of intensity, all infected PSAC had low infection intensity.
In our study, the odds of infection were higher among PSAC under the care of older caregivers. In rural South Africa, it is common for grandmothers to raise up to two generations of grandchildren . Older caregivers may not be able to actively and closely monitor infants relative to their younger counterparts thus potentially exposing children to activities with high risk of STH infection. In addition, the use of open water sources increased the odds of both schistosomiasis and STH infection. The use of safe water and improved sanitation are essential in preventing the re-emergence of helminth infections after successful treatment. However, such are rarely present in poverty-stricken rural communities [14, 24]. While improved sanitation is central in the control of soil-transmitted helminthiases, it may not be the case in the control of schistosomiasis . If transmission sites remain full of parasite infested water, the prevalence of schistosomiasis is likely to persist regardless of improvements in sanitation or hygiene practices . Literature suggests that behavioural modification strategies such as the provision and promotion of safe water usage, and hygiene practices are key in the control of schistosomiasis and STH transmission . Other researchers, however, stress the importance of integrating control strategies in order to enhance their effectiveness. For instance, behavioural change interventions may not be effective if water, sanitation and hygiene interventions are overlooked .
Schistosome infection was associated with water contact activities such as bathing PSAC in the river. Previous studies have shown that Schistosoma infection is more prevalent among PSAC whose parents/guardians have high contact with open water sources . In our study, predictors of STH infection were caregivers’ level of education, caregiver’s hand washing habit after toilet use and PSAC habit of washing hands with soap and water. Whilst treatment programmes are necessary to rapidly reduce the burden and morbidity of helminth infections, they are an unsustainable strategy for helminth control and for achieving control and elimination targets [3, 24, 28]. Progress towards achieving the elimination of schistosomiasis and soil-transmitted helminthiases crucially depends on sustainable resolutions that move beyond treating symptoms to reducing exposure. The prevention of reinfection is said to provide sustainable gains for schistosomiasis and soil-transmitted helminthiases control and elimination.
In our study, PSAC only provided single urine and stool samples from which schistosomiasis and STH infection were detected. The WHO encourages the collection of three stool/urine samples per child for accurate detection of infection. The collection of multiple samples also enables the assessment of intra and inter specimen variation of the egg output . Even though researchers were aware of these facts, the difficulty in obtaining multiple samples from children aged 1–5 years limited the implementation of this recommendation. We are also cognizant of the fact that in areas where the prevalence of soil-transmitted helminthiases is low, more sensitive techniques are required . While our findings are consistent with results from studies conducted among SAC in the study area showing a decline in the prevalence of schistosomiasis attributed to drought conditions that dried most open water bodies during the period 2015–2017 [7, 34], they may not be comparable with results from PSAC in rural settings with different climate conditions.
The prevalence of schistosomiasis in our study was low, suggesting that it may be possible to eliminate it among PSAC in the area provided vertical morbidity control interventions are integrated with community-wide health promotion strategies such as health education and the provision of clean water and basic sanitation. Our findings highlight the need for behavioural change interventions among caregivers of PSAC for schistosomiasis and soil-transmitted helminthiases control. The consideration of sociodemographic, water, sanitation and behavioural factors emerged as key in the success of soil-transmitted helminthiases and schistosomiasis control and elimination programmes. This is probably because the control of helminths largely depends on the progress towards addressing the root cause of infection beyond treating symptoms. Our study findings may add to the national health strategy for the inclusion of PSAC in NTD screening and treatment programmes. Findings also reveal contextual sociocultural gaps that may hinder existing control programmes from intensifying the control of schistosomiasis and soil-transmitted helminthiases and their possible elimination. The screening and treatment of PSAC could be integrated into existing child immunization programmes. Findings from the study recommended the inclusion of caregivers in schistosomiasis and soil-transmitted helminthiases control programmes.
Availability of data and materials
Data supporting the findings of this study are available on reasonable request from the corresponding author [HGS]. The data are not publicly available because the study is part of a bigger project (TIBASA–http://tiba-partnership.org/) that is still ongoing.
Acquired immune-deficiency syndrome
Analysis of Variance
- CI :
Early childhood development
Eggs per gram
Human immunodeficiency virus
Neglected tropical disease
- OR :
Preschool aged children
World Health Assembly
World Health Organization
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This research was commissioned by the National Institute of Health Research using Official Development Assistance (ODA) funding. The views expressed in this publication are those of the author(s) and not necessarily those of the NHSP, the National Institute of Health Research, or the Department of Health. The authors further acknowledge the KwaZulu-Natal Department of Health, uMkhanyakude district health office, Ingwavuma area community leaders and members who supported the study.
The study was mainly funded by the University of Edinburgh under the British Academy Project. The University of KwaZulu-Natal also supported the study through the College of Health Sciences Scholarship. HS is funded by the Medical Research Council of South Africa through a PhD scholarship under the National Health Scholars Program (NHSP). The funders had no role in designing this study, preparation of the manuscript and decision to have it published. Furthermore, the views, opinions, assumptions or any other information presented in this manuscript are solely those of the authors.
Ethics approval and consent to participate
The study was conducted after obtaining ethical approvals from the University of KwaZulu-Natal Biomedical Research Ethics Committee (BREC), Department of Health (DoH) and Provincial Health and Research Ethics Committee (PHREC); and the Department of Social Development in South Africa, KwaZulu-Natal province. Gatekeeper permission was also granted by uMkhanyakude district community leaders, primary schools, ECD centres and clinic facilities. Caregivers who participated in the study (parents and guardians) willingly consented and they also signed written consent forms on behalf of their children. The study gathered evidence useful to programmes and organization involved in the prevention and control of schistosomiasis and STH.
Consent for publication
The authors declare that they have no competing interests.
Multilingual abstracts in the five official working languages of the United Nations. (PDF 816 kb)